Research Article

Purification and Characterization of a New Lectin from Loach Skin Mucus

Figure 2

(a) Purity and molecular weight of LML by SDS-PAGE: lane markers: rainbow prestained markers (11 kDa-245 kDa); lane 1: crude extract, 2.5 mg/mL, 20 μL; lane 2: D-3 obtained by DEAE-52 ion-exchange chromatography, 1.4 mg/mL, 20 μL; lane 3: LML obtained by Sephadex G200 gel filtration chromatography, 1.0 mg/mL, 20 μL. (b) The electrophoretogram of LML by periodic acid-Schiff stain, 1.0 mg/mL, 20 μL.
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