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Experimental Diabetes Research
Volume 2012, Article ID 672013, 10 pages
http://dx.doi.org/10.1155/2012/672013
Research Article

Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells

1Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, China
2Department of Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226001, China

Received 24 December 2011; Revised 1 April 2012; Accepted 30 April 2012

Academic Editor: Joseph R. Landolph Jr.

Copyright © 2012 Guo Qing-Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [31 citations]

The following is the list of published articles that have cited the current article.

  • Saman Rahmati, Najva Alijani, and Mehdi Kadivar, “In vitro generation of glucose-responsive insulin producing cells using lentiviral based pdx-1 gene transduction of mouse (C57BL/6) mesenchymal stem cells,” Biochemical and Biophysical Research Communications, vol. 437, no. 3, pp. 413–419, 2013. View at Publisher · View at Google Scholar
  • Hiroshi Takemitsu, Dongwei Zhao, Shingo Ishikawa, Masaki Michishita, Toshiro Arai, and Ichiro Yamamoto, “Mechanism of insulin production in canine bone marrow derived mesenchymal stem cells,” General and Comparative Endocrinology, vol. 189, pp. 1–6, 2013. View at Publisher · View at Google Scholar
  • Ting-Ting Zhou, Chao Wei, Xiao-Dong Chen, Hai Li, and Zheng Wang, “Primary culture of umbilical cord-derived mesenchymal stem cells in serum free medium,” Chinese Journal of Tissue Engineering Research, vol. 17, no. 27, pp. 4980–4987, 2013. View at Publisher · View at Google Scholar
  • Vladislav Volarevic, Miodrag Stojkovic, and Majlinda Lakopp. 207–227, 2013. View at Publisher · View at Google Scholar
  • Devang M. Patel, Jainy Shah, and Anand S. Srivastava, “Therapeutic Potential of Mesenchymal Stem Cells in Regenerative Medicine,” Stem Cells International, vol. 2013, pp. 1–15, 2013. View at Publisher · View at Google Scholar
  • Xiang-Jun Fan, Lei Wang, Yu-Hua Lu, Mng-Yan Zhu, Hai-Xin Qian, and Zhi-Wei Wang, “Polygene-modified mouse induced differentiation of pluripotent stem cells into insulin-producing cells,” Academic Journal of Second Military Medical University, vol. 34, no. 7, pp. 700–707, 2013. View at Publisher · View at Google Scholar
  • Ashwini Ketkar-Atre, Tom Struys, Stefaan J. Soenen, Ivo Lambrichts, Catherine M. Verfaillie, Marcel De Cuyper, and Uwe Himmelreich, “Variability in contrast agent uptake by different but similar stem cell types,” International Journal of Nanomedicine, vol. 8, pp. 4577–4591, 2013. View at Publisher · View at Google Scholar
  • Phuc Van Pham, Phuoc Thi-My Nguyen, Anh Thai-Quynh Nguyen, Vuong Minh Pham, Anh Nguyen-Tu Bui, Loan Thi-Tung Dang, Khue Gia Nguyen, and Ngoc Kim Phan, “Improved differentiation of umbilical cord blood-derived mesenchymal stem cells into insulin-producing cells by PDX-1 mRNA transfection,” Differentiation, 2014. View at Publisher · View at Google Scholar
  • Weina Gao, Yuchang Fu, Cong Yu, Shunke Wang, Yuchao Zhang, Chen Zong, Tongfu Xu, Yong Liu, Xia Li, and Xiangdong Wang, “Elevation of NR4A3 Expression and Its Possible Role in Modulating Insulin Expression in the Pancreatic Beta Cell,” Plos One, vol. 9, no. 3, 2014. View at Publisher · View at Google Scholar
  • Jiang-Chao Li, Yongxia Yang, Xiao-Ming Zhou, Si-Mei Guo, Ying Liu, Cui-Ling Qi, Tian Lan, Qian-Qian Zhang, and Li-Jing Wang, “Micrometastasis expressing insulin arise in lung and spleen at advanced stage of Rip1-Tag2 transgenic mice,” International Journal of Biological Sciences, vol. 10, no. 2, pp. 136–141, 2014. View at Publisher · View at Google Scholar
  • Adam Nowakowski, Piotr Walczak, Miroslaw Janowski, and Barbara Lukomska, “Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine,” Stem Cells and Development, pp. 150826083522001, 2015. View at Publisher · View at Google Scholar
  • Anthony I. Romer, and Lori Sussel, “Pancreatic islet cell development and regeneration,” Current Opinion in Endocrinology, Diabetes and Obesity, vol. 22, no. 4, pp. 255–264, 2015. View at Publisher · View at Google Scholar
  • Marina Lizio, Yuri Ishizu, Masayoshi Itoh, Timo Lassmann, Akira Hasegawa, Atsutaka Kubosaki, Jessica Severin, Hideya Kawaji, Yukio Nakamura, Harukazu Suzuki, Yoshihide Hayashizaki, Piero Carninci, and Alistair R. R. Forrest, “Mapping Mammalian Cell-type-specific Transcriptional Regulatory Networks Using KD-CAGE and ChIP-seq Data in the TC-YIK Cell Line,” Frontiers in Genetics, vol. 6, 2015. View at Publisher · View at Google Scholar
  • Loan Thi-Tung Dang, Anh Nguyen-Tu Bui, Vuong Minh Pham, Ngoc Kim Phan, and Phuc Van Pham, “Production of islet-like insulin-producing cell clusters in vitro from adiposederived stem cells,” Biomedical Research and Therapy, vol. 2, no. 1, 2015. View at Publisher · View at Google Scholar
  • Wei Zhu, Chun-Gen Xing, Ming Chen, Ming Li, Shuyu Zhang, Jianping Cao, Aonan Du, Jing Xu, Yanchao Ma, Han Cao, Chao Yang, and Xiao-Dong Yang, “Neurogenic differentiation factor NeuroD confers protection against radiation-induced intestinal injury in mice,” Scientific Reports, vol. 6, 2016. View at Publisher · View at Google Scholar
  • Lan Xue, Wei Wang, Jian-Wen Xu, Lu Chen, Yan Liu, and Qiao-Mei Lin, “Electroacupuncture at Baihui (DU20) acupoint upregulates mRNA expression of NeuroD molecules in the brains of newborn rats suffering in utero fetal distress,” Neural Regeneration Research, vol. 11, no. 4, pp. 604–609, 2016. View at Publisher · View at Google Scholar
  • Zahra Mehrfarjam, Fariba Esmaeili, Leila Shabani, and Esmaeil Ebrahimie, “Induction of pancreatic β cell gene expression in mesenchymal stem cells,” Cell Biology International, vol. 40, no. 5, pp. 486–500, 2016. View at Publisher · View at Google Scholar
  • Maciej P Walczak, Anna M Drozd, Ewelina Stoczynska-Fidelus, Piotr Rieske, and Dawid P Grzela, “Directed differentiation of human iPSC into insulin producing cells is improved by induced expression of PDX1 and NKX6.1 factors in IPC progenitors.,” Journal of translational medicine, vol. 14, no. 1, pp. 341, 2016. View at Publisher · View at Google Scholar
  • Guido Sebastiani, Marco Valentini, Giuseppina Emanuela Grieco, Giuliana Ventriglia, Laura Nigi, Francesca Mancarella, Silvia Pellegrini, Gianvito Martino, Valeria Sordi, Lorenzo Piemonti, and Francesco Dotta, “MicroRNA expression profiles of human iPSCs differentiation into insulin-producing cells,” Acta Diabetologica, 2016. View at Publisher · View at Google Scholar
  • Xiao-Xu Ge, and Li-Mei Liu, “Research progresses of MafA gene mutations/variations and diabetes mellitus,” Journal of Shanghai Jiaotong University (Medical Science), vol. 36, no. 10, pp. 1513–1516, 2016. View at Publisher · View at Google Scholar
  • Nermin Abdel-hamid Sadik, Nadia Said Metwally, Olfat Gamil Shaker, Mahmoud Sanad Soliman, Ahmed Abdelaziz Mohamed, and Mai Mohamed Abdelmoaty, “Local Renin-Angiotensin System Regulates the Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells through Angiotensin Type 2 Receptor,” Biochimie, 2017. View at Publisher · View at Google Scholar
  • Ping Shi, Xiao-Yu Liu, Xue-Wen Lin, Xi-Ning Pang, Hong-Tu Li, Fang-Xu Jiang, Tao Zhang, and Zhong-Yan San, “In vitro reprogramming of rat bmMSCs into pancreatic endocrine-like cells,” In Vitro Cellular and Developmental Biology - Animal, vol. 53, no. 2, pp. 157–166, 2017. View at Publisher · View at Google Scholar
  • V. Sordi, S. Pellegrini, M. Krampera, P. Marchetti, A. Pessina, G. Ciardelli, G. Fadini, C. Pintus, G. Pantè, and L. Piemonti, “Stem cells to restore insulin production and cure diabetes,” Nutrition, Metabolism and Cardiovascular Diseases, 2017. View at Publisher · View at Google Scholar
  • Jun Tan, Lihua Zhou, Peng Xue, Guangying Dong, Yang Zhou, Guangsheng Wu, Hang Guo, and Qintao Wang, “The influence of diabetes mellitus on proliferation and osteoblastic differentiation of MSCs,” Current Stem Cell Research and Therapy, vol. 12, no. 5, pp. 388–400, 2017. View at Publisher · View at Google Scholar
  • Lu Liang, Jie Feng, Alain Rupin, Youwei Wang, Zhong Chao Han, Tao Ma, Jie Geng, Jimin Wang, Bingjing Zhang, Lanzhen Zhao, Zongjin Li, Zhibo Han, Wenjing Du, Honghong Jia, and Meng Zhao, “Transplantation of human placenta-derived mesenchymal stem cells alleviates critical limb ischemia in diabetic nude rats,” Cell Transplantation, vol. 26, no. 1, pp. 45–61, 2017. View at Publisher · View at Google Scholar
  • Liang-Liang Sun, Tian-Jin Liu, Limei Li, Wei Tang, Jun-Jie Zou, Xiang-Fang Chen, Jiao-Yang Zheng, Bei-Ge Jiang, and Yong-Quan Shi, “Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice,” International Journal of Molecular Medicine, vol. 39, no. 4, pp. 936–948, 2017. View at Publisher · View at Google Scholar
  • Ayşegül AÇIksari, Gökhan Duruksu, and Erdal KaraÖZ, “Improved insulin-secreting properties of pancreatic islet mesenchymalstem cells by constitutive expression of Pax4 and MafA,” Turkish Journal Of Biology, vol. 41, pp. 979–991, 2017. View at Publisher · View at Google Scholar
  • Mohammad Reza Tabandeh, Ata A. Ghadiri, Elham Allah Bakhshi, Mahmoud Orazizadeh, Dian Dayer, Eskandar Moghimipour, Mahmood Hashemi Tabar, and Mohammad Ali Ghafari, “MAFA overexpression: A new efficient protocol for in vitro differentiation of adipose-derived mesenchymal stem cells into functional insulin-producing cells,” Cell Journal, vol. 21, no. 2, pp. 169–178, 2018. View at Publisher · View at Google Scholar
  • Nie Lin, Qiang Ma, Rongping Chen, Zhen Zhang, Weiheng Wen, Jia Sun, Yuting Ruan, and Hong Chen, “Circulating LncRNAs Analysis in Patients with Type 2 Diabetes Reveals Novel Genes Influencing Glucose Metabolism and Islet β-Cell Function,” Cellular Physiology and Biochemistry, vol. 46, no. 1, pp. 335–350, 2018. View at Publisher · View at Google Scholar
  • Hui Shi, Hang Li, Weiying Zhang, Lihong Ye, Zhen Wang, Xiaodong Zhang, Yinghui Li, Runping Fang, and Huawei Wang, “Hepatitis B X-interacting protein promotes the formation of the insulin gene-transcribing protein complex Pdx-1/Neurod1 in animal pancreatic β-cells,” Journal of Biological Chemistry, vol. 293, no. 6, pp. 2053–2065, 2018. View at Publisher · View at Google Scholar
  • Dario Gerace, Rosetta Martiniello-Wilks, Rosaline Habib, Binhai Ren, Najah Therese Nassif, Bronwyn Anne O’Brien, and Ann Margaret Simpson, “ Ex Vivo Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic β -Cells ,” Stem Cells International, vol. 2019, pp. 1–15, 2019. View at Publisher · View at Google Scholar