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Journal of Diabetes Research
Volume 2016, Article ID 6571976, 22 pages
http://dx.doi.org/10.1155/2016/6571976
Research Article

Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics

1Department of Biomedical Sciences, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea
2Department of Biomedical Engineering, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea
3Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea
4Department of Biostatistics, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, 20 Borame-ro 5-gil, Dongjak-gu, Seoul 156-707, Republic of Korea
5Department of Ophthalmology, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Republic of Korea
6Department of Statistics, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-747, Republic of Korea

Received 10 November 2014; Accepted 5 March 2015

Academic Editor: Ying-Feng Zheng

Copyright © 2016 Jonghwa Jin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

For identification of protein, an enhanced mass scan (EMS) ranging from 400 to 1200 m/z (scan speed, 4000 Da/s) was performed as an first MRM step, and an enhanced product ion (EPI) scan was then carried out to obtain MS/MS spectra. A MASCOT search was performed using the MS/MS spectra to identify the protein.

Mascot.dll 1.6b23 and ABSciex.DataAccess.Wiff File DataReader.dll were used for importing data to Mascot and SwissProt 57.12 was used for database (513877 entries). The other database search parameters used for searching were the following: enzyme trypsin, 1 missed cleavage, variable carbamidomethylation modifications (C), oxidation (M), peptide tolerance 0.15 Da, MS/MS tolerance 0.1 Da, peptide charge 1+, and monoisotopic. Only significant hits at a p<0.05, as defined by MASCOT probability analysis, were accepted.

  1. Supplementary Material