Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling

Figure 2

1,25(OH)₂D₃ deficiency increases hepatic gluconeogenesis. (a) Blood glucose levels of 6-month-old male 1a(OH)ase^-/- and wild-type mice with rescued diet at different time points (fasting, fasting for 6 h, fasting for 24 h) ().; blue bar: RDWT group; red bar: RDKO group. (b) PTT was performed in 6-month-old 1α(OH)ase^-/- and wild-type mice (). ; blue line: RDWT group; red line: RDKO group. (c) qPCR was used to detect the mRNA expression levels of gluconeogenesis-related genes G6Pase and PCK1 in the liver of 1α(OH)ase^-/- mice and wild-type littermates (). (d) Western blotting was performed to detect the protein expression levels of G6Pase and PCK1 in the liver of the two groups of mice; blue bar: RDWT group; red bar: RDKO group. (e) GTT was performed in 6-month-old 1α(OH)ase^-/- and wild-type mice; blue line: RDWT group; red line: RDKO group; . (f) During the GTT, the serum in two groups of mice was collected at 0, 15, and 120 minutes, and the insulin content in the serum of 1α(OH)ase^-/- mice did not show significant difference as compared to the wild-type littermates; blue bar: RDWT group; red bar: RDKO group; . (g) Western blotting confirmed the siRNA interference efficiency in HepG2 cells. (h) Glucose oxidase method was used to detect the supernatant glucose content of two groups of cells, si-NC group and si-VDR group (). . (i, j) Western blotting and qRT-PCR detected the mRNA (j) and protein (i) expressions of G6Pase and PCK1 in the HepG2 cells with VDR knockdown (). ; blue bar: RDWT group; red bar: RDKO group; blue line: RDWT group; red line: RDKO group; blue bar: si-NC group; red bar: si-VDR group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)