Effects of Different Enzymes and Concentrations in the Production of Clarified Lemon Juice

Table 2

Pectinmethylesterase activity values of lemon juices (%).

Stages of production	Rapidase Intense			Novoferm 61			Novozym 33095			Factory production
	10; (μL/100 mL)	20; (μL/100 mL)	40; (μL/100 mL)	10; (μL/100 mL)	20; (μL/100 mL)	40; (μL/100 mL)	10; (μL/100 mL)	20; (μL/100 mL)	40; (μL/100 mL)	20; (μL/100 mL)
Extraction	100^a	100^a	100^a	100^a	100^a	100^a	100^a	100^a	100^a	100^a
Pulp removal	85.87 ± 1.92^b	85.87 ± 1.92^b	85.87 ± 1.92^b	81.77 ± 6.79^b	81.77 ± 6.79^b	81.77 ± 6.79^b	88.15 ± 7.66^b	88.15 ± 7.66^b	88.15 ± 7.66^b	9.95 ± 4.24^b
Pasteurization	15.64 ± 1.58^c	15.64 ± 1.58^c	15.64 ± 1.58^c	14.25 ± 1.25^cd	14.25 ± 1.25^c	14.25 ± 1.25^c	13.09 ± 2.64^c	13.09 ± 2.64^c	13.09 ± 2.64^c	1.61 ± 0.08^c
Enzymation	9.44 ± 4.39^dA	9.23 ± 0.84^dA	10.07 ± 1.58^dA	17.91 ± 4.83^cA	15.53 ± 1.18^cA	15.16 ± 1.75^cA	10.01 ± 1.23^cA	9.53 ± 0.61^cA	8.16 ± 1.42^cdA	1.79 ± 0.25^c
Clarification	8.13 ± 1.39^dA	9.55 ± 3.31^dA	6.82 ± 0.38^eA	13.07 ± 0.34^cdA	10.09 ± 3.91^cA	7.35 ± 2.14^dA	9.21 ± 1.41^cA	8.19 ± 0.61^cA	7.18 ± 0.81^cdA	1.52 ± 0.27^c
Rotary vacuum filter	—	—	—	—	—	—	—	—	—	2.19 ± 0.13^c
Kieselguhr filter	—	—	—	—	—	—	—	—	—	2.02 ± 0.19^c
Paper filter	8.06 ± 1.04^dA	7.46 ± 1.85^dA	5.29 ± 0.81^eA	9.22 ± 1.58^dA	9.80 ± 5.21^cA	6.82 ± 0.08^dA	7.65 ± 0.71^cA	8.05 ± 0.74^cA	6.09 ± 0.26^eA	1.85 ± 0.24^c

Small letters on the same column show the difference between the stages of production; uppercased letters in the same row show the difference between enzyme concentrations for each enzyme. The difference between stage of production and enzyme concentrations is at level of 0.01 (𝑃<0.01) importance.