Protease from Aspergillus oryzae: Biochemical Characterization and Application as a Potential Biocatalyst for Production of Protein Hydrolysates with Antioxidant Activities

Table 1

The central composite rotatable design (CCRD) matrix used to determine the optimum pH and temperature for activity and stability of the protease from A. oryzae LBA 01, with the coded and real values for the variables and responses.

Optimum pH and temperature for protease activity
Runs	Independent variables		Protease activity (U·g^−1)
	/pH	/temperature (°C)
1	−1 (5.3)	−1 (39.4)	2,151.34 ± 1.88
2	+1 (6.7)	−1 (39.4)	921.91 ± 17.83
3	−1 (5.3)	+1 (60.6)	3,961.30 ± 132.97
4	+1 (6.7)	+1 (60.6)	957.53 ± 1.88
5	−1.41 (5.0)	0 (50.0)	2,703.55 ± 2.50
6	+1.41 (7.0)	0 (50.0)	880.76 ± 18.46
7	0 (6.0)	−1.41 (35.0)	1,361.51 ± 61.32
8	0 (6.0)	+1.41 (65.0)	1,691.60 ± 91.36
9	0 (6.0)	0 (50.0)	2,863.29 ± 0.63
10	0 (6.0)	0 (50.0)	2,878.33 ± 30.04
11	0 (6.0)	0 (50.0)	2,989.39 ± 31.29
Optimum pH and temperature for protease stability
Runs	/pH	/temperature (°C)	Relative activity (%)
1	−1 (4.4)	−1 (39.4)	91.50 ± 3.07
2	+1 (6.6)	−1 (39.4)	71.94 ± 3.84
3	−1 (4.4)	+1 (60.6)	39.79 ± 4.97
4	+1 (6.6)	+1 (60.6)	0.32 ± 0.10
5	−1.41 (4.0)	0 (50.0)	57.71 ± 3.63
6	+1.41 (7.0)	0 (50.0)	17.98 ± 3.30
7	0 (5.5)	−1.41 (35.0)	100.00 ± 5.35
8	0 (5.5)	+1.41 (65.0)	0.59 ± 0.17
9	0 (5.5)	0 (50.0)	80.37 ± 2.80
10	0 (5.5)	0 (50.0)	80.47 ± 3.46
11	0 (5.5)	0 (50.0)	80.38 ± 3.92