Protease from Aspergillus oryzae: Biochemical Characterization and Application as a Potential Biocatalyst for Production of Protein Hydrolysates with Antioxidant Activities
Table 4
The effects of various salts and compounds on the protease activity.
Ions and inhibitors
Residual activity (%)
0.05 mM
0.5 mM
5.0 mM
Control
100.00 ± 4.57
ZnSO4
96.59 ± 7.63
55.41 ± 3.82
23.88 ± 2.44
MgSO4
97.10 ± 4.13
97.77 ± 4.10
97.21 ± 2.12
FeSO4
90.45 ± 5.09
42.96 ± 3.44
Undetected
MnSO4
105.99 ± 4.63
89.56 ± 4.67
80.87 ± 5.89
CaCl2
95.03 ± 5.11
92.73 ± 5.31
93.96 ± 1.56
CuSO4
82.68 ± 2.83
22.19 ± 0.61
13.98 ± 3.53
NaCl
99.39 ± 2.22
103.13 ± 2.01
100.29 ± 4.49
CoCl2
88.71 ± 0.67
72.72 ± 4.51
Undetected
(NH4)2SO4
100.66 ± 4.08
98.52 ± 3.34
95.73 ± 3.00
Glutathione
97.95 ± 3.97
97.29 ± 3.22
101.97 ± 3.36
Cysteine
98.26 ± 3.96
87.23 ± 2.76
46.80 ± 2.29
KCl
96.55 ± 4.93
91.62 ± 4.46
30.65 ± 4.77
Mg(NO3)2
98.01 ± 0.46
97.38 ± 3.16
94.31 ± 1.72
MnCl2
101.74 ± 3.93
92.17 ± 8.80
73.83 ± 7.17
CaCO3
101.65 ± 3.41
96.99 ± 1.36
52.01 ± 2.75
EDTA
101.52 ± 2.02
100.13 ± 3.47
99.25 ± 3.56
Iodoacetamide
79.98 ± 3.87
63.79 ± 2.51
33.20 ± 3.10
p-Chloromercuribenzoate
98.89 ± 4.55
97.52 ± 2.86
94.66 ± 2.47
Sodium azide
96.03 ± 0.26
97.20 ± 1.55
97.38 ± 5.20
N-Bromosuccinimide
78.56 ± 3.76
10.56 ± 2.12
5.10 ± 1.40
Urea
96.73 ± 2.99
94.70 ± 3.43
92.58 ± 3.20
Results are presented as the mean () ± SD (standard deviation).
