Application of Melting Temperature in Melting Curve of qPCR to Determine Listeria monocytogenes Presence in Golden Needle Mushroom

Table 2

Detection of the Listeria monocytogenes in the samples, which showed a negative or positive results in the conventional method Tm value.

Sample	Conventional method	Detection by Tm (°C)	Observation by plating after 3 h enrichment
A	−^(1)	79.0	−
B	−	84.3	+^(2)
C	−	84.5	+
D	−	84.5	+

⁽¹⁾−, negative; ⁽²⁾+, positive.