Determination of the Chemical Stability of Cyanocobalamin in Medical Food by a Validated Immunoaffinity Column-Linked HPLC Method

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td class="align_left">Parameters</td><td class="align_center">Conditions</td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left" rowspan="3">Column</td><td class="align_center">Agilent Poroshell BONUS RP (4.6 mm × 150 mm, 2.7 <i>μ</i>m)</td></tr><tr><td class="align_center">Agilent Poroshell EC-C18 (4.6 mm × 50 mm, 2.7 <i>μ</i>m)</td></tr><tr><td class="align_center">Agilent Poroshell SB-AQ (4.6 mm × 150 mm, 2.7 <i>μ</i>m)</td></tr><tr><td class="align_left" colspan="2"><hr/></td></tr><tr><td class="align_left">Oven temperature</td><td class="align_center">40°C</td></tr><tr><td class="align_left" rowspan="2">Flow rate</td><td class="align_center">Preseparation column: 0.3 mL/min</td></tr><tr><td class="align_center">Focusing/analytical column: 0.3 mL/min</td></tr><tr><td class="align_left" colspan="2"><hr/></td></tr><tr><td class="align_left">Injection volume</td><td class="align_center">150 <i>μ</i>L</td></tr><tr><td class="align_left" rowspan="2">Mobile phase</td><td class="align_center">Preseparation column: MeOH:5 mM KH<sub>2</sub>PO<sub>4</sub> (15 : 85, v/v)</td></tr><tr><td class="align_center">Focusing/analytical column: MeOH:5 mM KH<sub>2</sub>PO<sub>4</sub> (25 : 75, v/v)</td></tr><tr><td class="align_left" colspan="2"><hr/></td></tr><tr><td class="align_left">Wavelength</td><td class="align_center">361 nm</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>HPLC analysis condition for cyanocobalamin analysis.</div>

Journal of Food Quality

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Table 1

Table 1: Determination of the Chemical Stability of Cyanocobalamin in Medical Food by a Validated Immunoaffinity Column-Linked HPLC Method 