Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus

Figure 4

The detection sensitivity of the spt5 gene detection in qualitative PCR. The initial amount of DNA in each PCR reaction is as follows: 1-2, 40 ng; 3-4, 4 ng; 5-6, 0.4 ng; 7-8, 0.04 ng; 9-10, 0.004 ng; 11-12, 0.0004 ng; 13, negative control; M DNA marker DL 2000.