Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus

Figure 5

The detection sensitivity of the spt5 gene detection in SYBR Green I quantitative PCR. (a) The final content of DNA in each reaction is as follows: 1, 40 ng; 2, 4 ng; 3, 0.4 ng; 4, 0.04 ng. (b) The standard curve of the quantitative PCR.

(a)

(b)