Research Article
Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus
Table 1
Primers used in qualitative and quantitative PCR.
| | Primer name | Primer sequence (5′⟶3′) | Length | Product size (bp) | Reference |
| | ITS-F | TCCGTAGGTGAACCTGCGG | 19 | | This study | | ITS-R | TCCTCCGCTTATTGATATGC | 20 | | spt5-F | GGTCTTGTTGTGTCTGTTTCGG | 22 | 189 | | spt5-R | TTGCCTACAATGTTTGTGCCA | 21 |
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