Species-Specific Gene, spt5, in the Qualitative and Quantitative Detection of Boletus reticulatus

Table 2

PCR amplification system.

Reagent	Final concentration	Volume (μL)
10× buffer	1×	2.5
dNTPs	0.2 mM	2
Forward primer	0.4 μM	1
Reverse primer	0.4 μM	1
Taq DNA polymerase	2.5 units	0.2
DNA template	5 ng/μL	2
Water		16.3
Total volume		25