Genomic Diversity and Virulence Genes Characterization of Bacillus cereus sensu lato Isolated from Processing Equipment of an Algerian Dairy Plant

Table 1

Genes, primers sequences and PCR conditions used in this study.

Gene	Primer	Sequence (5′-3′)	Product (bp)	Cycles	Denat.	Anneal.	Elong.	Ref
Sph	Ph1	CGT GCC GAT TTA ATT GGG GC	558	35	20 s, 94°C	20 s, 58°C	20 s, 72°C	[42]
	Ph2	CAA TGT TTT AAA CAT GGA TGC G
bceT	ETF	TTA CAT TAC CAG GAC GTG CTT	428	35	20 s, 94°C	20 s, 56°C	20 s, 72°C	[43]
	ETR	TGT TTG TGA TTG TAA TTC AGG
entFM	ENTA	ATG AAA AAA GTA ATT TGC AGG	1269	35	20 s, 94°C	20 s, 52°C	20 s, 72°C	[44]
	ENTB	TTA GTA TGC TTT TGT GTA ACC
entS	TY123	GGT TTA GCA GCA GCT TCT GTA GCT GGC G	581	29	40 s, 94°C	60 s, 60.7°C	60 s, 72°C	[44]
	TY125	GTT TCG TTA GAT ACA GCA GAA CCA CC
pipIc	PC105	CGC TAT CAA TGG ACC ATG G	569	30	30 s, 94°C	45 s, 57°C	90 s, 72°C	[45]
	PC106	GGA CTA TTC CAT GCT GTA CC
cytK	F2	AAC AGA TAT CGG TCA AAA TGC	623	30	60 s, 94°C	60 s, 52°C	60 s, 72°C	[26]
	R7	CGT GCA TCT GTT TCA T
nheA	344S	TAC GCT AAG GAG GGG CA	499	30	15 s, 94°C	45 s, 55°C	120 s, 72°C	[25]
	843A	GTT TTT ATT GCT TCA TCG GCT
nheB	1500S	CTA TCA GCA CTT ATG GCA G	769	30	15 s, 94°C	45 s, 55°C	120 s, 72°C	[25]
	2269A	ACT CCT AGC GGT GTT CC
nheC	2820S	CGG TAG TGA TTG CTG GG	581	30	15 s, 94°C	45 s, 55°C	120 s, 72°C	[25]
	3401A	CAG CAT TCG TAC TTG CCA A
hblC	FHC	GAA TGG TCA TCG GAA CTC TA	672	30	60 s, 94°C	60 s, 54°C	60 s, 72°C	[41]
	RHC	CAT CAG GTC ATA CTC TTG TGT
Ces	cesF1	GGT GAC ACA TTA TCA TAT AAG GTG	1.271	35	30 s, 94°C	45 s, 58°C	90 s, 72°C	[23]
	cesR2	GTA AGC GAA CCT GTC TGT AAC AAC A