Effect of Adding Thymus leptobotrys on Extra Virgin Olive Oil’s Quality and Physicochemical Parameters

Table 5

UHPLC/DAD/ESI-MS of control olive oil and enriched with powder and essential oil of T. leptobotrytis.

N°	RT	HPLC/UV	m/z (M-H)-	Fragments	Proposed compound	EVOO (%)	VOOP (%)		VOOEO (%)
							2.5%	5%	100 ppm	200 ppm
1	7.05	210, 280	153.20	111/97.18/79.2	Hydroxy-tyrosol	4.780 ± 0.126	0.120 ± 0.002	0.1000 ± 0.001	3.77 ± 0.226	3.82 ± 0.091
2	9.53	280	318.91	217/177.04	Decarboxymethyl oleuropein aglycone	8.61 ± 0.13	5.17 ± 0.09	4.08 ± 0.27	6.85 ± 0.19	6.90 ± 0.11
3	18.97	280	318.95	217/177.03	Decarboxymethyl oleuropein aglycone isomer	17.59 ± 0.15	10.26 ± 0.49	6.90 ± 0.35	14.85 ± 0.31	13.54 ± 0.04
4	21.24	222, 276	377	291/259	Oleuropeine aglycone	22.45 ± 0.44	15.96 ± 0.05	8.80 ± 0.04	18.91 ± 0.48	17.68 ± 0.08
5	21.8	220, 276	361.15	291/259/217/177	Ligstroside aglycone	4.42 ± 0.34	3.13 ± 0.05	3.57 ± 0.14	4.34 ± 0.44	3.99 ± 0.37
6	24.2	220, 276	319.08	307/275/217.13	Oxidized product of aldehydic form of ligstroside aglycone	6.58 ± 0.17	5.41 ± 0.04	4.55 ± 0.14	5.53 ± 0.17	4.85 ± 0.14
7	25.62	220, 276	319.03	307/275/217	Oxidized product of aldehydic form of ligstroside aglycone isomer	5.60 ± 0.24	3.80 ± 0.14	2.96 ± 0.12	4.84 ± 0.22	4.07 ± 0.01
8	26.45	280	285.17	—	Luteolin	13.12 ± 0.39	11.63 ± 0.07	9.94 ± 0.34	12.00 ± 0.39	11.43 ± 0.06
9	26.90	220, 276	318.91	307/275/217	Oxidized product of aldehydic form of ligstroside aglycone isomer	6.63 ± 0.19	5.00 ± 0.04	4.30 ± 0.03	5.59 ± 0.19	5.28 ± 0.01
10	27.27	236, 336	269.16	—	Apigenin	4.83 ± 0.11	2.11 ± 0.06	1.63 ± 0.12	3.04 ± 0.11	2.43 ± 0.03
11	27.65	244	329.38	311/291	Thymusin	—	32.13 ± 0.04	47.88 ± 0.80	14.89 ± 3.24	20.60 ± 0.69

Note: : Peak area %.