Characterization of Lactic Acid Bacteria and Pathogens Isolated from Traditionally Fermented Foods, In Relation to Food Safety and Antimicrobial Resistance in Tribal Hill Areas of Northeast India

Table 2

Details of PCR primers for identification and detection of functional and AMR genes.

General functions	Gene	Primer		AT	References
		Orientation	Sequence 5′ to 3′	(°C)
Survival pH	hdc	F	AGA​TGG​TAT​TGT​TTC​TTA​TG	52	[34]
		R	AGA​CCA​TAC​ACC​ATA​ACC​TT
Bile salt	Bsh	F	ATTGAAGGCGGAACSGGMTA	58
		R	ATWACCGGWCGGAAAGCTG
Synthesis of B vitamins	folP	F	CCASGRCSGCTTGCATGAC	59.5
		R	TKACGCCGGACTCCTTTTWY
	folK	F	CCA​TTT​CCA​GGT​GGG​GAA​TC	59.5
		R	GGG​GTG​GTC​CAA​GCA​AAC​TT
Riboflavin synthesis	ribH	F	AGG​GCG​AAA​CCG​ACC​ACT​AC	60
		R	CGA​TTG​GGC​AGT​CAT​CGA​AC
	ribB	F	AGT​AAA​CGG​AAC​GGG​CAA​GC	60
		R	GTT​GAC​CAG​GGC​ACC​AAC​TG
	ribA	F	TTT​ACG​GGC​GAT​GTT​TTA​GG	60
		R	CGA​CCC​TCT​TGC​CGT​AAA​TA
Starch metabolism	α-amy	F	AGA​TCA​GGC​GCA​AGT​TCA​GT	60
		R	TTT​TAT​GGG​CAC​ACC​ACT​CA
Tetracycline	tetA	F	GTA​ATT​CTG​AGC​ACT​GTC​GC	57	[35]
		R	CTG​TCC​TGG​ACA​ACA​TTG​CTT
	tetB	F	CTC​AGT​ATT​CCA​AGC​CTT​TG	52
		R	CTA​AGC​ACT​TGT​CTC​CTG​TT
Quinolone	qnrA	F	GGATGCCAGTTTCGAGGA	59	[36]
		R	TGCCAGGCACAGATCTTG
	qnrB	F	GGMATHGAAATTCGCCACTG	55	[37]
		R	TTTGCYGYYCGCCAGTCGAA
β-Lactamase	blaTEM	F	ATG​AGT​ATT​CAA​CAT​TTC​CG	55	[38]
		R	TTA​ATC​AGT​GAG​GCA​CCT​AT
	CTX-M	F	CGCTTTGCGATGTGCAG	60
		R	ACCGCGATATCGTTGGT

AT; annealing temperature.