Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding

Table 1

Comparison of DNA concentration and purity results obtained for canned tuna samples (n = 24) extracted in duplicate with four different DNA extraction methods.

	DNA concentration (ng/µl)	A260/280	A260/230
DNeasy Blood and Tissue Kit	35.32 ± 37.03^a	1.20 ± 0.09^a	0.83 ± 0.47^a
DNeasy Blood and Tissue Kit plus PowerClean Kit	17.85 ± 8.17^b	1.55 ± 1.12^b	0.98 ± 1.18^a
MP FastPrep plus NucleoSpin Tissue Kit	13.91 ± 25.67^c	1.33 ± 0.31^b	0.62 ± 0.51^b
DNeasy Mericon Food Kit	16.98 ± 8.43^b	1.78 ± 0.33^c	0.66 ± 0.22^a

^abcA different superscript letter in the same column indicates a significant difference, according to a Kruskal–Wallis H test with the post hoc Dunn’s test (). Values are expressed as the average ± standard deviation.