Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding

Table 3

Sequencing success results obtained for the four different categories of canned tuna separated by DNA extraction methods.

DNA extraction methods	Sequencing success rate
	Light tuna (%)	Albacore tuna (%)	Skipjack tuna (%)	Yellowfin tuna (%)
DNeasy Blood and Tissue Kit	16.7	83.3	0	66.7
DNeasy Blood and Tissue Kit plus PowerClean Kit	0	66.7	0	16.7
MP FastPrep plus NucleoSpin Tissue Kit	16.7^a	66.7	16.7^b	16.7
DNeasy Nericon Food Kit	0	100	16.7^b	50
Results of all kits combined	33.3	100	16.7	83.3

^aLabeled as “light tuna,” sequencing identified as albacore (Thunnus alalunga). ^bLabeled as “skipjack tuna,” sequencing identified as albacore (T. alalunga) and yellowfin (Thunnus albacares). A total of six samples were tested per canned tuna category. Samples were considered successful if at least one of the duplicate extractions showed sequencing results.