Journal of Food Quality / 2023 / Article / Tab 4 / Research Article
Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding Table 4 Comparison of labor, modifications, price, and time for the four DNA extraction kits used.
DNA extraction kits Protocol Manufacturer Modifications Ease of use Hands-ontechnician time (h) DNA extraction price per sample (USD)a Estimated time (h) required to extract 15 samples DNeasy Blood and Tissue Kit Spin-column animal tissue Qiagen Elution volume decreased Easy moderate 1 $5.27 21 DNeasy Blood and Tissue Kit plus PowerClean Kit Spin-column animal tissue (DNeasy) + detailed (PowerClean) Qiagen Elution volume decreased; AE buffer used to elute Moderate-difficult 2 $10.37 22 MP FastPrep plus NucleoSpin Tissue Kit Standard protocol for animal tissue MP-Biomedicals; Machery-Nagel Speed 6 for 40 sec; elution volume decreased Moderate 1.5 $6.01 4.5 DNeasy Mericon Food Kit Small fragment (200 mg) Qiagen Elution volume decreased Moderate 1.5 $5.83 2
a Price per sample is based on the list price for each kit (50 extractions per kit) at the manufacturer’s website plus additional reagents required. Prices do not include the cost of instrumentation or consumables (e.g., pipet tips or extraction tubes).