Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding

Table 4

Comparison of labor, modifications, price, and time for the four DNA extraction kits used.

DNA extraction kits	Protocol	Manufacturer	Modifications	Ease of use	Hands-ontechnician time (h)	DNA extraction price per sample (USD)^a	Estimated time (h) required to extract 15 samples
DNeasy Blood and Tissue Kit	Spin-column animal tissue	Qiagen	Elution volume decreased	Easy moderate	1	$5.27	21
DNeasy Blood and Tissue Kit plus PowerClean Kit	Spin-column animal tissue (DNeasy) + detailed (PowerClean)	Qiagen	Elution volume decreased; AE buffer used to elute	Moderate-difficult	2	$10.37	22
MP FastPrep plus NucleoSpin Tissue Kit	Standard protocol for animal tissue	MP-Biomedicals; Machery-Nagel	Speed 6 for 40 sec; elution volume decreased	Moderate	1.5	$6.01	4.5
DNeasy Mericon Food Kit	Small fragment (200 mg)	Qiagen	Elution volume decreased	Moderate	1.5	$5.83	2

^aPrice per sample is based on the list price for each kit (50 extractions per kit) at the manufacturer’s website plus additional reagents required. Prices do not include the cost of instrumentation or consumables (e.g., pipet tips or extraction tubes).