Determination of 60 Food-Borne Stimulant Drug Residues in Animal-Derived Foods by Solid-Phase Extraction Purification and Ultra-High-Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry

Table 2

Linearity, limits of detection (LOD), limits of quantitation (LOQ), and matrix effect (ME) of 60 food-borne stimulants.

No.	Compound	Internal standard compound	Linear equation	Linear range (ng/mL)		LOD (μg/kg)	LOQ (μg/kg)	ME
1	Clenbuterol	Clenbuterol-D9	Y = 0.0328466 + 0.10541X	0.5–40	0.9976	0.1	0.2	0.85
2	Salbutamol	Salbutamol-D3	Y = 0.00695222 + 0.0827112X	0.5–40	0.9983	0.1	0.2	0.90
3	Ractopamine	Ractopamine-D3	Y = 0.00962176 + 0.0887182X	0.25–20	0.9997	0.05	0.1	0.93
4	Terbutaline	Salbutamol-D3	Y = 0.0563537 + 0.062133X	0.5–40	0.9972	0.1	0.2	0.92
5	Fenoterol	Clenbuterol-D9	Y = −0.00131536 + 0.045025X	0.25–20	0.9979	0.05	0.1	0.82
6	Tulobuterol	Clenbuterol-D9	Y = 0.0403942 + 0.142663X	0.25–20	0.9992	0.05	0.1	0.75
7	Penbutolol	Clenbuterol-D9	Y = −0.0634425 + 0.296814X	0.5–40	0.9980	0.1	0.2	0.78
8	Cimaterol	Clenbuterol-D9	Y = 0.0233392 + 0.0250413X	0.5–40	0.9981	0.1	0.2	0.81
9	Salmeterol	Clenbuterol-D9	Y = 0.0254088 + 0.11891X	0.25–20	0.9976	0.05	0.1	0.84
10	Atenolol	Salbutamol-D3	Y = −0.0294185 + 0.315163X	0.5–40	0.9988	0.1	0.2	0.78
11	Metoprolol	Clenbuterol-D9	Y = 0.0805915 + 0.247612X	0.25–20	0.9980	0.05	0.1	0.89
12	Clenproperol	Ractopamine-D3	Y = −0.0188051 + 0.0436501	0.5–40	0.9988	0.1	0.2	0.77
13	Demethyl coclaurine	Demethyl Coclaurine-D4	Y = −0.00387219 + 0.0814972X	0.5–40	0.9995	0.1	0.2	0.88
14	Tretoquinol	Tretoquinol-D9	Y = −0.0679088 + 0.156311X	0.5–40	0.9987	0.1	0.2	0.90
15	Propanolol	Clenbuterol-D9	Y = 0.056474 + 0.285512X	0.25–20	0.9977	0.05	0.1	0.78
16	Meprednisone	Hydrocortisone-D2	Y = −0.0727716 + 0.1656X	0.5–40	0.9989	0.1	0.2	0.74
17	Prednisolone	Hydrocortisone-D2	Y = −0.00815719 + 0.0527182X	0.5–40	0.9992	0.1	0.2	0.86
18	Methylprednisolone	Hydrocortisone-D2	Y = −0.0237101 + 0.0520901X	0.5–40	0.9992	0.1	0.2	0.68
19	Dexamethasone	Hydrocortisone-D2	Y = −0.0114097 + 0.0332118X	0.5–40	0.9988	0.1	0.2	0.77
20	Betamethasone	Hydrocortisone-D2	Y = −0.02721 + 0.0441686X	0.5–40	0.9987	0.1	0.2	0.80
21	Beclomethasone	Hydrocortisone-D2	Y = −0.0222696 + 0.0337372X	0.5–40	0.9989	0.1	0.2	0.70
22	Fludrocortisone	Hydrocortisone-D2	Y = −0.0364505 + 0.180234X	0.5–40	0.9988	0.1	0.2	0.63
23	Hydrocortisone	Hydrocortisone-D2	Y = −0.0287757 + 0.155345X	0.5–40	0.9995	0.1	0.2	0.89
24	Cortisone	Hydrocortisone-D2	Y = −0.0318001 + 0.0787036X	0.5–40	0.9991	0.1	0.2	0.88
25	Zilpaterol	Salbutamol-D3	Y = 0.0081897 + 0.0555902X	0.5–40	0.9973	0.1	0.2	0.88
26	Stanozolol	Testosterone-D3	Y = 0.295443 + 0.652478X	0.5–40	0.9975	0.1	0.2	0.78
27	Trenbolone	Testosterone-D3	Y = 0.0899871 + 0.331158X	0.25–20	0.9974	0.05	0.1	0.65
28	Metandienone	Testosterone-D3	Y = 0.0148753 + 0.10149X	0.5–40	0.9982	0.1	0.2	0.69
29	17-Methyltestosterone	Testosterone-D3	Y = 0.0434338 + 0.117486X	0.5–40	0.9969	0.1	0.2	0.71
30	Testosterone	Testosterone-D3	Y = 0.0121521 + 0.10279X	0.5–40	0.9985	0.1	0.2	0.93
31	Nandrolone	Testosterone-D3	Y = 0.015237 + 0.0979825X	0.5–40	0.9986	0.1	0.2	0.58
32	Testosterone propionate	Testosterone-D3	Y = 0.0044069 + 0.0430173X	0.5–40	0.9996	0.1	0.2	0.91
33	Nandrolone 17-propionate	Testosterone-D3	Y = 0.0195716 + 0.031933X	0.5–40	0.9985	0.1	0.2	0.81
34	Boldenone	Testosterone-D3	Y = 0.0253621 + 0.0937769X	0.5–40	0.9974	0.1	0.2	0.69
35	Nandrolone phenylpropionate	Testosterone-D3	Y = −0.0364081 + 0.0300981X	0.25–20	0.9967	0.05	0.1	1.18
36	Dehydroepiandrosterone	Testosterone-D3	Y = 0.0121521 + 0.10279X	0.5–40	0.9985	0.1	0.2	0.70
37	Zeranol	Zeranol-D5	Y = −0.00666451 + 0.10036X	0.5–40	0.9988	0.1	0.2	0.75
38	Beta-zearalanol	Zeranol-D5	Y = −0.0100097 + 0.080692X	0.5–40	0.9993	0.1	0.2	0.68
39	Alpha-zearalenol	Zeranol-D5	Y = 0.00132244 + 0.131453X	0.5–40	0.9983	0.1	0.2	0.77
40	Beta-zearalenol	Zeranol-D5	Y = −0.00197718 + 0.099839X	0.5–40	0.9984	0.1	0.2	0.80
41	Zearalanone	Zeranol-D5	Y = 0.019878 + 0.204666X	0.5–40	0.9984	0.1	0.2	0.75
42	Zearalenone	Zeranol-D5	Y = −0.0401997 + 0.253658X	0.5–40	0.9994	0.1	0.2	0.69
43	Acetazolamide	Furosemide-D5	Y = 0.00503859 + 0.0917645X	2.5–200	0.9983	0.5	1	0.93
44	Canrenone	Canrenone-D6	Y = 0.0261506 + 0.165535X	2.5–200	0.9992	0.5	1	0.86
45	Chlortalidone	Bendroflumethiazide-D5	Y = −0.00787723 + 0.0278848X	2.5–200	0.9985	0.5	1	0.83
46	Furosemide	Furosemide-D5	Y = −0.000751913 + 0.0762856X	2.5–200	0.9993	0.5	1	0.92
47	Spironolactone	Canrenone-D6	Y = 0.00130985 + 0.0250254X	2.5–200	0.9992	0.5	1	0.82
48	Bendroflumethiazide	Bendroflumethiazide-D5	Y = 0.00187415 + 0.0851601X	2.5–200	0.9981	0.5	1	0.90
49	Chlorothiazide	Bendroflumethiazide-D5	Y = 0.0158104 + 0.101733X	2.5–200	0.9996	0.5	1	1.10
50	Hydrochlorothiazide	Bendroflumethiazide-D5	Y = −0.00456868 + 0.0353866X	2.5–200	0.9985	0.5	1	1.08
51	Triamterene	Canrenone-D6	Y = 0.479403 + 0.829713X	2.5–200	0.9973	0.5	1	0.61
52	4-Amino-6-chlorobenzene-1,3-disulfonamide	Furosemide-D5	Y = −0.00539682 + 0.109846X	2.5–200	0.9989	0.5	1	0.65
53	Bumetanide	Canrenone-D6	Y = −0.0387555 + 0.0476122	2.5–200	0.9987	0.5	1	0.70
54	Torasemide	Canrenone-D6	Y = −0.0583651 + 0.0986786X	2.5–200	0.9988	0.5	1	0.78
55	Acebutolol	Acebutolol-D7	Y = −0.00480551 + 0.0829962X	0.5–40	0.9999	0.1	0.2	0.93
56	Bromoclenbuterol	Clenbuterol-D9	Y = −0.0492107 + 0.0921308X	0.5–40	0.9978	0.1	0.2	0.88
57	Celipolol	Celipolol-D9	Y = 0.000804285 + 0.099369X	0.5–40	1.0000	0.1	0.2	0.95
58	Formoterol	Clenbuterol-D9	Y = −0.127637 + 0.14402X	0.5–40	0.9976	0.1	0.2	0.85
59	Brombuterol	Clenbuterol-D9	Y = −0.0463769 + 0.0683862X	0.5–40	0.9966	0.1	0.2	0.80
60	Nebivolol	Nebivolol-D4	Y = −0.00563137 + 0.26977X	0.5–40	0.9987	0.1	0.2	0.90