Detection of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in Inactivated Fermented Milk Using Fluorescence Quantitative Loop-Mediated Isothermal Amplification

Table 4

Reproducibility results of the limit of detection of L. bulgaricus in fermented milk using FQ-LAMP.

Order number	Concentration (CFU/g)	Times number	Mean Ct	SD	CV (%)
1	8.1 × 10^8	5	11.277	0.425	3.78
2	8.1 × 10^7	5	13.428	0.393	2.93
3	8.1 × 10^6	5	15.777	0.532	3.38
4	8.1 × 10^5	5	18.640	0.723	3.88
5	8.1 × 10^4	5	20.651	0.957	4.64
6	8.1 × 10^3	5	22.334	1.182	5.29
7	8.1 × 10^2	5	26.582	1.36	5.12

Note. 1–7: these 10-fold serial dilutions of L. bulgaricus were analysed using FQ-LAMP.