Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells
Figure 2
Genetic engineering of CIK cells with CARs. (a) Schematic diagram of the expression cassettes coding for the CEA-specific CAR BW431/26scFv-Fc-CD3ζ and BW431/26scFv-Fc-CD28-CD3ζ used in this study. (b) CIK cells were generated from mononuclear cells and subsequently transduced by retroviral infection to express the anti-CEA CAR. Mock-transduced cells (w/o) served as controls. CAR expression was recorded by flow cytometry using the anti-CD3 antibody OKT3 and the anti-human IgG-Fc antibody which detects the CAR extracellular IgG1 Fc spacer domain.
