Table of Contents Author Guidelines Submit a Manuscript
Clinical and Developmental Immunology
Volume 2012 (2012), Article ID 238924, 8 pages
Research Article

Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells

1Department of Neurosurgery, University of Bonn, 53127 Bonn, Germany
2Center for Molecular Medicine Cologne and Department I of Internal Medicine, University of Cologne, 53901 Cologne, Germany
3Center for Integrated Oncology Cologne-Bonn, Germany
4Department of Internal Medicine III, University Hospital Bonn, Sigmund-Freud-Straße 25, 53105 Bonn, Germany

Received 2 September 2011; Revised 3 November 2011; Accepted 17 November 2011

Academic Editor: Ana Lepique

Copyright © 2012 Claudia Schlimper et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adoptive therapy of malignant diseases with cytokine-induced killer (CIK) cells showed promise in a number of trials; the activation of CIK cells from cancer patients towards their autologous cancer cells still needs to be improved. Here, we generated CIK cells ex vivo from blood lymphocytes of colorectal cancer patients and engineered those cells with a chimeric antigen receptor (CAR) with an antibody-defined specificity for carcinoembryonic antigen (CEA). CIK cells thereby gained a new specificity as defined by the CAR and showed increase in activation towards CEA+ colon carcinoma cells, but less in presence of CEA cells, indicated by increased secretion of proinflammatory cytokines. Redirected CIK activation was superior by CAR-mediated CD28-CD3ζ than CD3ζ signaling only. CAR-engineered CIK cells from colon carcinoma patients showed improved activation against their autologous, primary carcinoma cells from biopsies resulting in more efficient tumour cell lysis. We assume that adoptive therapy with CAR-modified CIK cells shows improved selectivity in targeting autologous tumour lesions.

1. Introduction

Although a variety of therapeutic options for metastatic colon cancer were evaluated during the last decade, most patients in advanced stages of the disease have no hope for cure by standard therapies. Alternative therapeutic approaches including immunotherapy are currently explored [1]. One of the major pitfalls in the adoptive immunotherapy of cancer is the strikingly low activation of T cells from cancer patients compared to healthy donors due to reduced expression of TCR/CD3 components [2]. The need for alternative effector cells in targeting colorectal carcinoma becomes obvious by the fact that T cells infiltrating colon cancer metastases have reduced CD3ζ chain expression and lack tumour-specific activation [3]. Compared to firstly activated effector T cells, ex vivo generated cytokine-induced killer (CIK) cells have a number of advantages since they exhibit properties different from effector or central memory T cells, that is, CIK cells are activated in an MHC-independent fashion [4, 5], produce proinflammatory cytokines, mainly IFN-γ and IL-4 [6, 7], and exhibit antigen-independent cytolytic activities against a variety of tumour cells. CIK cells are generated ex vivo by extensive stimulation of CD3+ CD56- CD8+ T cells with IFN-γ and CD3 and prolonged propagation in presence of high-dose IL-2 [4]. After 2-3 weeks in culture, the majority of cells exhibit a large granular lymphocyte morphology and express both NK and T-cell markers including CD8, CD11a, CD49d, CD56, and NKG2D, while lacking most NK-cell-associated activating and inhibitory receptors [8]. The CD45RA+ CCR7 CD62L(+), CD27+, CD28, MIF-1a+ CIK phenotype coincides with that for terminally differentiated memory T cells [9]. CIK cells display extraordinary cytolytic capacities toward a broad array of malignant cells [10] and traffic efficiently to the tumour side after systemic delivery [11]. Upon activation, CIK cells upregulate perforin and FasL as well as DAP10 which couples NKG2D signaling to perforin-based cytotoxicity [12], thereby recognizing a class of stress-associated ligands, NKG2D ligands, expressed on the tumour cell surface. Consequently, CIK cells exhibit MHC-unrestricted cytotoxicity and do not rely on a particular antigen. Based on these and other properties, CIK cells attracted interest for adoptive immunotherapy particularly in advanced stages of the disease where repression of MHC expression or defects in the antigen-processing machinery frequently occur. For application in adoptive therapy, CIK cells display the advantage that they do not require priming but can rapidly be expanded in culture [13] and are less associated with graft-versus-host disease than conventional effector T cells [14]. CIK cells have been adoptively transferred in phase I trials to treat leukemia/lymphoma and various solid tumours including hepatocellular carcinoma, colon carcinoma, astrocytoma, melanoma, and renal cell carcinoma [1517]. CIK therapy showed low toxicity [18], however, limited therapeutic efficacy; CIK therapy is consequently assumed to require large numbers of CIK cells to be transferred to achieve efficient tumour clearance.

In this situation, we asked to improve CIK cell activation against autologous tumour cells. We therefore made use of the concept to redirect T cells towards defined target cells by a recombinant chimeric antigen receptor (CAR) which is expressed on the surface of T cells and provides both antigen-targeting specificity and T-cell activation [19]. The CAR in the extracellular moiety is composed of a single-chain fragment of variable region (scFv) antibody for target binding and in the intracellular moiety of the CD3ζ signaling chain to initiate T-cell activation upon binding. To furthermore increase T-cell activation, the costimulatory CD28 endodomain was linked to CD3ζ in a combined signaling moiety [20]. We here demonstrate that ex vivo generated CIK cells from colon carcinoma patients can be engineered with a tumour-specific CAR; such “designer” CIK cells increase cytokine secretion and cytolysis when engaging autologous, primary colon carcinoma cells. Data suggest such CAR-engineered CIK cells to improve the antitumour response in the adoptive immunotherapy of colon carcinoma.

2. Materials and Methods

2.1. Patient Characteristics and Evaluation

Patients with colorectal carcinoma were treated by surgery of the primary tumour lesion. Approval of the local ethics committee was obtained. Diagnosis of CEA+ colorectal carcinoma was confirmed by immunohistology in a pathology reference centre.

2.2. Cells, Cell Lines, and Reagents

T cells were isolated from heparinized peripheral blood by Ficoll density centrifugation. CIK cells were generated as previously described [21]. In brief, nonadherent peripheral blood mononuclear cells were stimulated in RPMI 1640 medium, 10% (v/v) FCS, and 25 mM HEPES with human recombinant IFN-γ (1,000 U/mL; Roche Biochemicals, Mannheim, Germany). After 24 h, 50 ng/mL OKT3 monoclonal antibody (mAb) (Orthoclone; Cilag, Sulzbach, Germany), 100 U/mL IL-1β, and 300 U/mL IL-2 (Roche, Mannheim, Germany) were added. Cells were propagated in a density of  cells/mL in presence of IL-2. Primary colon carcinoma cell cultures were established from patients' carcinoma specimens obtained during surgery as described [22]. In brief, tissue specimens were incubated in HBSS buffer containing 100 U/mL DNase I (Roche Biochemicals), 50 U/mL collagenase III (Biochrom, Berlin, Germany), 150 U/mL hyaluronidase (Sigma, Deisenhofen, Germany), and 0,08 U/mL insulin (Hoechst, Bad Soden a. Ts., Germany) at 37°C for 15 min. Cells in the supernatant were centrifuged for 5 min at 400 × g and erythrocytes eliminated by incubation in 10 mL “erythrocyte-lysis buffer” (8,29 g/L NH4Cl, 1 g/L KHCO3, 0,0371 g/L EDTA) and for 15 min. Cells were washed and resuspended in Leibovitz medium, 10% (v/v) FCS, 1 mM L-glutamine, 1x MEM vitamins, 2.5 mg/mL transferrin, 1 g/L sodium bicarbonate, 1 g/L glucose, 80 U/mL insulin, and 10 mg/mL gentamycin (all from Gibco Invitrogen, Karlsruhe, Germany). Cultures contaminated with fibroblasts were removed. Carcinoma cells grown in culture were monitored for CEA expression by immunohistochemical analysis using an anti-CEA mAb 1C3 (Abcam, Cambridge, MA) and a peroxidase-conjugated Fab anti-mouse Ab (1 : 50) (Roche Diagnostics) and visualized by 3-Amino-9-ethylcarbazole (AEC; Sigma). 293T cells are human embryonic kidney cells that express the SV40 large T antigen. LS174T is a CEA+ colorectal carcinoma line (ATCC, CL-188), and Colo201 is a CEA- adenocarcinoma line (ATCC CCL 224). OKT3 (ATCC CRL 8001) is a hybridoma cell line that produces the anti-CD3 mAb OKT3. 293T cells were propagated in DMEM medium supplemented with 10% (v/v) FCS, and all other cell lines were cultured in RPMI 1640 medium, 10% (v/v) FCS (all Life Technologies, Paisly, UK). OKT3 monoclonal antibody (mAb) was affinity purified from hybridoma supernatants utilizing goat anti-mouse IgG2a antibodies (Southern Biotechnology, Birmingham, AL, USA) that were immobilized on N-hydroxy-succinimid-ester-(NHS)-activated sepharose (Amersham Biosciences, Freiburg, Germany). Human IgG1 antibodies and the phycoerythrin-(PE-)conjugated anti-CD3 mAb UCHT1 were purchased from Dako, Hamburg, Germany, and the goat antihuman IgG antibody and its FITC- and PE-conjugated F(ab′)2 derivatives from Southern Biotechnology. The antihuman IFN-γ mAb NIB42 and the biotinylated anti-human IFN-γ mAb 4S.B3 were purchased from BD Bioscience, San Diego, CA, USA.

2.3. Engineering of CIK Cells and Receptor-Mediated Activation

The generation of the expression cassettes for the CEA-specific CARs BW431/26-scFv-Fc-ζ (no. 439) and BW431/26-scFv-Fc-CD28-ζ (no. 607) was previously described [20]. CIK cells were engineered with the CAR by retroviral gene transfer, and CAR expression was identified by flow cytometry utilizing a PE- or FITC-conjugated F(ab′)2 anti-human IgG1 antibody (1 μg/mL), which recognizes the CAR extracellular IgG1 Fc spacer, and the anti-CD3 mAb (UCHT-1, 1 : 20). Flow cytometry was performed using an FACScan cytofluorometer equipped with the CellQuest research software (Becton Dickinson, Mountain View, CA, USA). Engineered CIK cells were cocultivated in round bottom 96-well microtiter plates (1.25−10 × 104 engineered cells/well) with CEA+ and CEA tumour cells (5 × 104 cells/well), respectively. After 48 hrs, culture supernatants were analyzed by ELISA for IFN-γ. Briefly, IFN-γ was bound to the solid phase anti-human IFN-γ mAb NIB42 (1 μg/mL) and detected by the biotinylated anti-human IFN-γ mAb 4S.B3 (0.5 μg/mL) (both from Pharmingen). The reaction product was visualized by a peroxidase-streptavidin conjugate (1 : 10,000) and ABTS (both from Roche Diagnostics).

2.4. Cytotoxicity Assay

Specific cytotoxicity of receptor-grafted T cells against target cells was monitored by an XTT-based colorimetric assay. Briefly, XTT (2,3-bis(2-methoxy-4-nitro-5sulphonyl)-5[(phenyl-amino)carbonyl]-2H-tetrazolium hydroxide) reagent (1 mg/mL) (“Cell Proliferation Kit II,” Roche Diagnostics) was added to the cells and incubated for 30–90 min at 37°C. Reduction of XTT to formazan by viable tumour cells was monitored colorimetrically at an adsorbance wavelength of 450 nm and a reference wavelength of 650 nm. Maximal reduction of XTT was determined as the mean of wells containing tumour cells only, and the background as the mean of wells containing culture medium only. The nonspecific formation of formazan due to the presence of effector cells was determined from triplicate wells containing effector cells in the same number as in the corresponding experimental wells. The cytotoxicity towards tumour cells was calculated as follows: cytotoxicity [%] = 100 − {[OD (exp. wells-corresponding number of effector cells)/OD (tumour cells without effectors − medium)] × 100}.

2.5. ELISpot Assay

IFN-γ-producing cells were determined using the human “IFN-γ ELISpot kit” (Hölzel, Cologne, Germany). Peripheral blood lymphocytes (  cells) were plated on nitrocellulose 96-well plates (Millipore, Bedford, MA) coated with anti-IFN-γ antibody. Cells were stimulated either with phytohemagglutinin (10 μg/mL, Sigma) or with 100 Gy irradiated tumour cells ( tumour cells per CIK cells) for 48 hrs. Bound IFN-γ was detected by a biotinylated antibody and visualized by streptavidin alkaline phosphatase and BCIP/NBT (BioRad, Munich, Germany) as substrate. Spots were recorded using the Bioreader 2000 (Bio Sys, Karben, Germany).

2.6. Statistical Analyses

Statistical analyses were performed using the two-tailed Student's -test if not otherwise described.

3. Results

CD3+ CD56+ CIK cells were generated in vitro from peripheral blood lymphocytes of a healthy donor by incubation with IFN-γ, IL-1beta, and the agonistic anti-CD3 mAb OKT3 and propagated in the presence of IL-2 as previously described [21]. After 2-3 weeks, CIK cells showed their characteristic repertoire of surface molecules (Table 1). CIK cells are activated upon coincubation with CEA+ Colo205 and CEA Colo201 colon carcinoma cells, respectively, indicated by the increase in the number of IFN-γ-secreting CIK cells (Figure 1). Activation of CIK cells occurred equally upon binding to CEA+ and CEA tumour cells confirming the known property of CIK cells of antigen-independent antitumour activation.

Table 1: Characterization of CIK cells.
Figure 1: CIK cells are equally activated upon binding to CEA+ and CEA tumour cells. CIK cells ( cells) generated from blood lymphocytes of healthy donors were coincubated with CEA+ Colo205 or CEA Colo201 tumour cells (103 tumour cells) for 48 hrs. IFN-γ production was monitored by ELIspot analysis. Data represent the mean ± standard error of the mean; a representative experiment out of three is shown. * .

We asked to furthermore improve CIK cell activation selectively towards CEA+ colorectal carcinoma cells by engineering with a CEA-specific CAR. Therefore, CIK cells were retrovirally transduced to express either the anti-CEA CAR BW431/26scFv-Fc-CD3ζ with the CD3ζ or alternatively the anti-CEA CAR BW431/26scFv-Fc-CD28-CD3ζ with the combined CD28-CD3ζ signaling domain, both harboring the same CEA binding domain (Figure 2(a)). Both CARs were efficiently expressed on the CIK cell surface as recorded by flow cytometry using an antibody directed towards the CAR extracellular IgG1 Fc spacer domain (Figure 2(b)). CAR expression on engineered cells was similar on CIK cells from healthy donors and from colorectal cancer patients.

Figure 2: Genetic engineering of CIK cells with CARs. (a) Schematic diagram of the expression cassettes coding for the CEA-specific CAR BW431/26scFv-Fc-CD3ζ and BW431/26scFv-Fc-CD28-CD3ζ used in this study. (b) CIK cells were generated from mononuclear cells and subsequently transduced by retroviral infection to express the anti-CEA CAR. Mock-transduced cells (w/o) served as controls. CAR expression was recorded by flow cytometry using the anti-CD3 antibody OKT3 and the anti-human IgG-Fc antibody which detects the CAR extracellular IgG1 Fc spacer domain.

In order to record antigen-redirected activation, CIK cells with engineered anti-CEA CAR were coincubated with CEA+ colon carcinoma cells and with CEA carcinoma cells as controls. CAR CIK cells increased IFN-γ secretion upon coincubation with CEA+, but not upon co-incubation with CEA- tumour cells, whereas CIK cells without CAR did not increase IFN-γ secretion (Figure 3(a)). IFN-γ secretion was more increased when CIK cells were activated by the CD28-CD3ζ than the CD3ζ CAR. Moreover, redirected cytolytic activity of CAR-engineered CIK cells towards CEA+ tumour cells is increased compared to CIK cells without CAR. The cytolytic activity towards CEA tumour cells was not substantially altered by engineering with a CEA-specific CAR as control. We conclude that CIK cells can specifically be improved in activation by CAR engagement.

Figure 3: CAR engagement produces higher activation of engineered CIK cells toward colorectal carcinoma cells. (a) CIK cells were engineered with the CEA-specific ζ or CD28-ζ CAR (1–  CAR+ cells/well) and incubated for 48 hrs with CEA+ LS174T and CEA Colo201 cells (5 × 104 cells/well). Mock-modified CIK cells without CAR (w/o) served as control. IFN-γ secreted by activated CIK cells into the culture supernatants was recorded by ELISA (upper), and cytolysis of tumour cells was monitored using the XTT-based viability assay (lower). * compared to nonmodified CIK cells (w/o). (b) To block the CEA-specific CAR binding domain, CIK cells with ζ CAR were incubated in presence of the anti-idiotypic antibody BW2064, which binds to the CAR BW431/26-scFv domain, or with an isotype-matched IgG1 control antibody of irrelevant specificity together with CEA+ LS174T tumour cells for 48 hrs. IFN-γ secreted into the culture supernatant and specific cytotoxicity toward LS174T cells were monitored. Data show a representative experiment out of three. * .

To confirm antigen specificity in CAR-mediated CIK activation, we blocked the CAR by incubation with the anti-idiotypic antibody BW2064, which is directed toward the BW431/26-scFv domain for antigen binding. As summarized in Figure 3(b), both IFN-γ secretion and cytolysis of CAR CIK cells were repressed in presence of the anti-idiotypic mAb, whereas an isotype-matched IgG1 antibody of irrelevant specificity had no effect. Data demonstrate that the antitumour activation of CIK cells is mediated by the engineered CAR in a CEA-dependent fashion.

We now explored whether activation of CIK cells from colorectal cancer patients toward their autologous tumour cells can be improved by CAR-mediated engagement of target cells. Colorectal carcinoma cells were isolated from surgical specimens and confirmed by immunostainings to express CEA (data not shown). Colorectal carcinoma cells were coincubated with engineered autologous CIK cells with the CD3ζ or the combined CD28-CD3ζ CAR. As summarized in Figure 4, activation of CAR CIK cells is substantially increased against autologous tumour cells compared to nonmodified CIK cells of the same patient. Increase in IFN-γ secretion was higher upon stimulation by the CD28-CD3ζ than by the CD3ζ signaling CAR. Increased IFN-γ secretion is due to increased numbers of activated CIK cells as indicated by the increased numbers of IFN-γ-ELISpots. Activation is antigen specific since the same CAR-engineered CIK cells did not increase IFN-γ secretion in presence of CEA Colo201 cells (data not shown). Taken together activation of CIK cells from colorectal cancer patients toward autologous tumour cells can be improved by CAR engagement of target cells and is furthermore increased by combined CD28-CD3ζ CAR signaling.

Figure 4: Activation of CIK cells from cancer patients against autologous colon carcinoma cells. Primary colon carcinoma cells were isolated from a colon carcinoma biopsy as described in Section 2 and cultured in vitro for short term. CIK cells from the same patient were generated in vitro and engineered with the ζ and the CD28-ζ CAR. CIK cells were coincubated for 48 hrs with the autologous CEA+ colon carcinoma cells ( CIK cells; 103 tumour cells). Secreted IFN-γ in the culture supernatant was monitored by ELISA. Data represent the means of triplicates ± standard error of the mean. One representative experiment out of three is shown. * .

4. Discussion

To improve CIK cell activation towards autologous tumour cells, we here revealed that (i) CIK cells generated ex vivo from peripheral blood lymphocytes can be engineered with a CAR as a targeting and activating receptor, (ii) engineered CIK cells increase activation upon CAR engagement which is superior upon CD28-CD3ζ signaling, and (iii) redirected by the CAR, CIK cells from tumour patients exhibit improved activation towards autologous tumour cells. While CIK cells recognize tumour cells in an antigen-independent fashion, CAR-engineered CIK cells gain antigen specificity as defined by the CAR binding domain. CAR-engineered CIK cells show improved tumour specificity indicated by increased IFN-γ secretion upon contact to CEA+ compared to CEA tumour cells, while nonmodified CIK cells do not increase IFN-γ in presence of CEA+ compared to CEA tumour cells. Improved CIK cell activation requires antigen engagement since activation is blocked by an anti-idiotypic antibody directed against the CAR binding domain. While CIK cells are susceptible to CD3ζ signaling, furthermore increase in IFN-γ secretion by designer CIK cells upon CD28-ζ CAR stimulation likely contributes to improve antitumour activity in vivo through activation of bystander cells. Cytolytic activity is predominantly mediated by perforin triggered by NKG2D in CIK cells [12]; other mechanisms may additionally contribute since blocking of NKG2D did not completely eliminate the cytotoxic activity of CIK cells [23].

To evaluate the specific situation in cancer patients, we confronted CIK cells from a colon cancer patient with the autologous colon cancer cells from a biopsy in vitro. Similarly as CIK cells from a healthy donor, CAR-engineered CIK cells from cancer patients showed improved activation against autologous tumour cells indicated by increase in IFN-γ secretion compared to CIK cells without CAR. Previous reports by Sheen et al. [24] and our group [25] demonstrated efficient targeting of CD3+ effector T cells from cancer patients toward autologous colon carcinoma cells; CIK cells herewith expand the panel of effector cells suitable to target autologous tumour cells.

Soluble CEA in the serum of cancer patients, particularly in advanced stages of the disease, may prevent CAR-mediated activation of engineered CIK cells by blocking the binding domain. In the case of the BW431/26 scFv CAR domain, we previously demonstrated that CEA in concentrations up to 20 μg/mL does not block CAR-mediated T-cell activation and does not inhibit induction of cytolytic activities [26]. We therefore do not expect that serum CEA interferes with the activity of anti-CEA CAR-modified CIK cells in colorectal cancer patients.

Given the insufficiencies in activating CIK cells and the difficulties in generating sufficient quantities for clinical applications, improved CIK activation upon CAR signaling is assumed to decrease the numbers of CIK cells required to elicit a therapeutic response. Previous strategies to overcome limitations in specific T-cell activation used bispecific antibodies which target CD3 on effector cells and the tumour-associated antigen CA125, Her2/neu, or other tumour-associated antigens on tumour cells [27, 28]. Redirecting CIK cells from patients with ovarian cancer with bispecific antibodies increased lysis of primary ovarian cancer cells [27]. Most recently, CIK cells were modified with a CD33-specific CAR for targeting acute myeloid leukemia cells [29] and with a CD19-specific CAR with 4-1BB costimulatory signal for targeting B-lineage acute lymphoblastic leukemia [29, 30].

5. Conclusion

CAR-mediated redirection of CIK cells from colon carcinoma patients improves their activation towards autologous tumour cells in an antigen-dependent fashion.


The authors thank Angela Märten for advice and discussions and Petra Alpmann for technical assistance. The work was funded by a grant from the Deutsche Krebshilfe, Bonn, Germany.


  1. M. S. Mitchell, “Cancer vaccines, a critical review-part I,” Current Opinion in Investigational Drugs, vol. 3, no. 1, pp. 140–149, 2002. View at Google Scholar · View at Scopus
  2. R. M. Bukowski, P. Rayman, R. Uzzo et al., “Signal transduction abnormalities in T lymphocytes from patients with advanced renal carcinoma: clinical relevance and effects of cytokine therapy,” Clinical Cancer Research, vol. 4, no. 10, pp. 2337–2347, 1998. View at Google Scholar · View at Scopus
  3. K. F. Yoong and D. H. Adams, “Interleukin 2 restores CD3-ζ chain expression but fails to generate tumour-specific lytic activity in Tumour-Infiltrating Lymphocytes derived from human Colorectal Hepatic Metastases,” British Journal of Cancer, vol. 77, no. 7, pp. 1072–1081, 1998. View at Google Scholar · View at Scopus
  4. I. G. Schmidt-Wolf, R. S. Negrin, H. P. Kiem, K. G. Blume, and I. L. Weissman, “Use of a SCID mouse/human lymphoma model to evaluate cytokine-induced killer cells with potent antitumor cell activity,” Journal of Experimental Medicine, vol. 174, no. 1, pp. 139–149, 1991. View at Google Scholar · View at Scopus
  5. D. Sangiolo, G. Mesiano, F. Carnevale-Schianca, W. Piacibello, M. Aglietta, and A. Cignetti, “Cytokine induced killer cells as adoptive immunotherapy strategy to augment graft versus tumor after hematopoietic cell transplantation,” Expert Opinion on Biological Therapy, vol. 9, no. 7, pp. 831–840, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. T. Yoshimoto and W. E. Paul, “CD4 positive, NK1.1 positive T cells promptly produce interleukin-4 in response to in vivo challenge with anti-CD3,” Journal of Experimental Medicine, vol. 179, no. 4, pp. 1285–1295, 1994. View at Google Scholar · View at Scopus
  7. C. Prussin and B. Foster, “TCR Valpha24 and Vbeta11 coexpression defines a human NK1 T cell analog containing a unique Th0 subpopulation,” The Journal of Immunology, vol. 159, pp. 5862–5870, 1997. View at Google Scholar
  8. M. R. Verneris, J. Baker, M. Edinger, and R. S. Negrin, “Studies of ex vivo activated and expanded CD8+ NK-T cells in humans and mice,” Journal of Clinical Immunology, vol. 22, no. 3, pp. 131–136, 2002. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Franceschetti, A. Pievani, G. Borleri et al., “Cytokine-induced killer cells are terminally differentiated activated CD8 cytotoxic T-EMRA lymphocytes,” Experimental Hematology, vol. 37, pp. 616–628, 2009. View at Google Scholar
  10. Y. C. Linn, L. C. Lau, and K. M. Hui, “Generation of cytokine-induced killer cells from leukaemic samples with in vitro cytotoxicity against autologous and allogeneic leukaemic blasts,” British Journal of Haematology, vol. 116, no. 1, pp. 78–86, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. V. Marin, E. Dander, E. Biagi et al., “Characterization of in vitro migratory properties of anti-CD19 chimeric receptor-redirected CIK cells for their potential use in B-ALL immunotherapy,” Experimental Hematology, vol. 34, no. 9, pp. 1219–1229, 2006. View at Google Scholar
  12. M. R. Verneris, B. Karami, J. Baker, A. Jayaswal, and R. S. Negrin, “Role of NKG2D signaling in the cytotoxicity of activated and expanded CD8+ T cells,” Blood, vol. 103, no. 8, pp. 3065–3072, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. I. Schmidt-Wolf, P. Lefterova, B. A. Mehta et al., “Phenotypic characterization and identification of effector cells involved in tumor cell recognition of cytokine-induced killer cells,” Experimental Hematology, vol. 21, no. 13, pp. 1673–1679, 1993. View at Google Scholar · View at Scopus
  14. R. Nishimura, J. Baker, A. Beilhack et al., “In vivo trafficking and survival of cytokine-induced killer cells resulting in minimal GvHD with retention of antitumor activity,” Blood, vol. 112, no. 6, pp. 2563–2574, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. T. Leemhuis, S. Wells, C. Scheffold, M. Edinger, and R. S. Negrin, “A phase I trial of autologous cytokine-induced killer cells for the treatment of relapsed Hodgkin disease and non-Hodgkin lymphoma,” Biology of Blood and Marrow Transplantation, vol. 11, no. 3, pp. 181–187, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Takayama, T. Sekine, M. Makuuchi et al., “Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial,” Lancet, vol. 356, no. 9232, pp. 802–807, 2000. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Introna, G. Borleri, E. Conti et al., “Repeated infusions of donor-derived cytokine-induced killer cells in patients relapsing after allogeneic stem cell transplantation: a phase I study,” Haematologica, vol. 92, no. 7, pp. 952–959, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Baker, M. R. Verneris, M. Ito, J. A. Shizuru, and R. S. Negrin, “Expansion of cytolytic CD8+ natural killer T cells with limited capacity for graft-versus-host disease induction due to interferon γ production,” Blood, vol. 97, no. 10, pp. 2923–2931, 2001. View at Publisher · View at Google Scholar · View at Scopus
  19. Z. Eshhar, “The T-body approach: redirecting T cells with antibody specificity,” Handbook of Experimental Pharmacology, no. 181, pp. 329–342, 2008. View at Google Scholar
  20. A. Hombach, A. Wieczarkowiecz, T. Marquardt et al., “Tumor-specific T cell activation by recombinant immunoreceptors: CD3ζ signaling and CD28 costimulation are simultaneously required for efficient IL-2 secretion and can be integrated into one combined CD28/CD3ζ signaling receptor molecule,” Journal of Immunology, vol. 167, no. 11, pp. 6123–6131, 2001. View at Google Scholar · View at Scopus
  21. B. Zoll, P. Lefterova, M. Csipai et al., “Generation of cytokine-induced killer cells using exogenous interleukin-2, -7 or -12,” Cancer Immunol Immunother, vol. 47, no. 4, pp. 221–226, 1998. View at Google Scholar
  22. B. Trojaneck, S. Niemitz, B. Micka et al., “Establishment and characterization of colon carcinoma and renal cell carcinoma primary cultures,” Cancer Biotherapy and Radiopharmaceuticals, vol. 15, no. 2, pp. 169–174, 2000. View at Google Scholar · View at Scopus
  23. Y. C. Linn, S. K. Lau, B. H. Liu, L. H. Ng, H. X. Yong, and K. M. Hui, “Characterization of the recognition and functional heterogeneity exhibited by cytokine-induced killer cell subsets against acute myeloid leukaemia target cell,” Immunology, vol. 126, pp. 423–435, 2009. View at Publisher · View at Google Scholar
  24. A. J. Sheen, J. Irlam, N. Kirillova et al., “Gene therapy of patient-derived T lymphocytes to target and eradicate colorectal hepatic metastases,” Diseases of the Colon and Rectum, vol. 46, no. 6, pp. 793–804, 2003. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Hombach, C. Schlimper, E. Sievers et al., “A recombinant anti-carcinoembryonic antigen immunoreceptor with combined CD3ζ-CD28 signalling targets T cells from colorectal cancer patients against their tumour cells,” Gut, vol. 55, no. 8, pp. 1156–1164, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. A. Hombach, D. Koch, R. Sircar et al., “A chimeric receptor that selectively targets membrane-bound carcinoembryonic antigen (mCEA) in the presence of soluble CEA,” Gene Therapy, vol. 6, no. 2, pp. 300–304, 1999. View at Publisher · View at Google Scholar · View at Scopus
  27. J. K. Chan, C. A. Hamilton, M. K. Cheung et al., “Enhanced killing of primary ovarian cancer by retargeting autologous cytokine-induced killer cells with bispecific antibodies: a preclinical study,” Clinical Cancer Research, vol. 12, no. 6, pp. 1859–1867, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Flieger, P. Kufer, I. Beier, T. Sauerbruch, and I. G. H. Schmidt-Wolf, “A bispecific single-chain antibody directed against EpCAM/CD3 mediates efficient cytotoxicity by cytokine-induced killer cells against EpCAM expressing tumor cells,” Cancer Immunology Immunotherapy, vol. 49, no. 8, pp. 441–448, 2000. View at Google Scholar
  29. V. Marin, I. Pizzitola, V. Agostoni et al., “Cytokine-induced killer cells for cell therapy of acute myeloid leukemia: improvement of their immune activity by expression of CD33-specific chimeric receptors,” Haematologica, vol. 95, no. 12, pp. 2144–2152, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. V. Marin, H. Kakuda, E. Dander et al., “Enhancement of the anti-leukemic activity of cytokine induced killer cells with an anti-CD19 chimeric receptor delivering a 4-1BB-ζ activating signal,” Experimental Hematology, vol. 35, no. 9, pp. 1388–1397, 2007. View at Publisher · View at Google Scholar · View at Scopus