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Clinical and Developmental Immunology
Volume 2012, Article ID 651058, 7 pages
http://dx.doi.org/10.1155/2012/651058
Research Article

Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

1Institute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, Germany
2Institute for Neuro- and Bioinformatics, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany

Received 5 October 2012; Accepted 18 October 2012

Academic Editor: Dimitrios P. Bogdanos

Copyright © 2012 Jörn Voigt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [37 citations]

The following is the list of published articles that have cited the current article.

  • F. Ouertani, H. Amiri, J. Bettaib, R. Yazidi, and A. Ben Salah, “Adaptive automatic segmentation of leishmaniasis parasite in indirect immunofluorescence images,” 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, pp. 4731–4734, . View at Publisher · View at Google Scholar
  • Chung-Chuan Cheng, Tsu-Yi Hsieh, Jin-Shiuh Taur, and Yung-Fu Chen, “An automatic segmentation and classification framework for anti-nuclear antibody images,” Biomedical Engineering Online, vol. 12, 2013. View at Publisher · View at Google Scholar
  • Nicola Bizzaro, Antonio Antico, Stefan Platzgummer, Elio Tonutti, Danila Bassetti, Fiorenza Pesente, Renato Tozzoli, Marilina Tampoia, and Danilo Villalta, “Automated antinuclear immunofluorescence antibody screening: A comparative study of six computer-aided diagnostic systems,” Autoimmunity Reviews, 2013. View at Publisher · View at Google Scholar
  • Daniel Bertin, Noemie Jourde-Chiche, Pierre Bongrand, and Nathalie Bardin, “Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence,” Clinical & Developmental Immunology, 2013. View at Publisher · View at Google Scholar
  • Sofie Schouwers, Myriam Bonnet, Patrick Verschueren, Rene Westhovens, Daniel Blockmans, Godelieve Marien, and Xavier Bossuyt, “Value-added reporting of antinuclear antibody testing by automated indirect immunofluorescence analysis,” Clinical Chemistry and Laboratory Medicine, vol. 52, no. 4, pp. 547–551, 2014. View at Publisher · View at Google Scholar
  • Francesca Buzzulini, Amelia Rigon, Paolo Soda, Leonardo Onofri, Maria Infantino, Luisa Arcarese, Giulio Iannello, and Antonella Afeltra, “The classification of Crithidia luciliae immunofluorescence test (CLIFT) using a novel automated system,” Arthritis Research and Therapy, vol. 16, 2014. View at Publisher · View at Google Scholar
  • Pier Luigi Meroni, Nicola Bizzaro, Ilaria Cavazzana, Maria Orietta Borghi, and Angela Tincani, “Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations,” Bmc Medicine, vol. 12, 2014. View at Publisher · View at Google Scholar
  • E. Csernok, “Laboratory Diagnostics of Connective Tissue Diseases and Vasculitides,” Aktuelle Rheumatologie, vol. 39, no. 1, pp. 49–57, 2014. View at Publisher · View at Google Scholar
  • Edda Rohwäder, Michael Locke, Johanna Fraune, and Kai Fechner, “Diagnostic profile on the IFA 40: HEp-20-10 – an immunofluorescence test for reliable antinuclear antibody screening,” Expert Review of Molecular Diagnostics, pp. 1–12, 2014. View at Publisher · View at Google Scholar
  • O. Shovman, N. Agmon-Levin, B. Gilburd, T. Martins, A. Petzold, T. Matthias, and Y. Shoenfeld, “A fully automated IIF system for the detection of antinuclear antibodies and antineutrophil cytoplasmic antibodies,” Immunologic Research, 2014. View at Publisher · View at Google Scholar
  • Chelsea Bentow, Gabriella Lakos, Rachel Rosenblum, Cassandra Bryant, Andrea Seaman, and Michael Mahler, “Clinical performance evaluation of a novel, automated chemiluminescent immunoassay, QUANTA Flash CTD Screen Plus,” Immunologic Research, 2014. View at Publisher · View at Google Scholar
  • Francesca Buzzulini, Amelia Rigon, Paolo Soda, Leonardo Onofri, Maria Infantino, Luisa Arcarese, Giulio Iannello, and Antonella Afeltra, “The classification of Crithidia luciliae immunofluorescence test (CLIFT) using a novel automated system,” Arthritis Research & Therapy, vol. 16, no. 2, 2014. View at Publisher · View at Google Scholar
  • Susan S. Copple, Troy D. Jaskowski, Rashelle Giles, and Harry R. Hill, “Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy,” Journal of Immunology Research, vol. 2014, pp. 1–7, 2014. View at Publisher · View at Google Scholar
  • Johannes Schulte-Pelkum, Antonella Radice, Gary L. Norman, Marcos Lόpez Hoyos, Gabriella Lakos, Carol Buchner, Lucile Musset, Makoto Miyara, Laura Stinton, and Michael Mahler, “Novel Clinical and Diagnostic Aspects of Antineutrophil Cytoplasmic Antibodies,” Journal of Immunology Research, vol. 2014, pp. 1–12, 2014. View at Publisher · View at Google Scholar
  • Michael Mahler, Pier-Luigi Meroni, Xavier Bossuyt, and Marvin J. Fritzler, “Current Concepts and Future Directions for the Assessment of Autoantibodies to Cellular Antigens Referred to as Anti-Nuclear Antibodies,” Journal of Immunology Research, vol. 2014, pp. 1–18, 2014. View at Publisher · View at Google Scholar
  • Chung-Chuan Cheng, Chun-Feng Lu, Tsu-Yi Hsieh, Yaw-Jen Lin, Jin-Shiuh Taur, and Yung-Fu Chen, “Design of a Computer-Assisted System to Automatically Detect Cell Types Using ANA IIF Images for the Diagnosis of Autoimmune Diseases,” Journal of Medical Systems, vol. 39, no. 10, 2015. View at Publisher · View at Google Scholar
  • C. Krause, K. Ens, K. Fechner, J. Voigt, J. Fraune, E. Rohwaeder, M. Hahn, M. Danckwardt, C. Feirer, E. Barth, T. Martinetz, and W. Stoecker, “EUROPattern Suite technology for computer-aided immunofluorescence microscopy in autoantibody diagnostics,” Lupus, vol. 24, no. 4-5, pp. 516–529, 2015. View at Publisher · View at Google Scholar
  • Daniel Bertin, Yassine Mouhajir, Pierre Bongrand, and Nathalie Bardin, “ICARE improves antinuclear antibody detection by overcoming the barriers preventing accreditation,” Clinica Chimica Acta, 2015. View at Publisher · View at Google Scholar
  • Luigi Cinquanta, Nicola Bizzaro, Danilo Villalta, Gabriella Morozzi, Elio Tonutti, Marcello Bagnasco, Giampaola Pesce, Danila Bassetti, Brunetta Porcelli, Lucia Terzuoli, Antonio Antico, Antonella Radice, Gaia Deleonardi, Marilina Tampoia, and Renato Tozzoli, “Linee guida per l’utilizzo dei test autoanticorpali nella diagnosi e nel monitoraggio delle malattie autoimmuni reumatiche sistemiche. Revisione 2015,” La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine, 2015. View at Publisher · View at Google Scholar
  • Stefan Gerlach, Kai Affeldt, Lena Pototzki, Christopher Krause, Jörn Voigt, Johanna Fraune, and Kai Fechner, “ Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology ,” Journal of Immunology Research, vol. 2015, pp. 1–8, 2015. View at Publisher · View at Google Scholar
  • Johanna Fraune, Stefan Gerlach, Kristin Rentzsch, Bianca Teegen, Sabine Lederer, Kai Affeldt, Kai Fechner, Maick Danckwardt, Jörn Voigt, Christian Probst, Lars Komorowski, and Winfried Stöcker, “Multiparametric serological testing in autoimmune encephalitis using computer-aided immunofluorescence microscopy (CAIFM),” Autoimmunity Reviews, 2016. View at Publisher · View at Google Scholar
  • Mandy Sowa, Rico Hiemann, Peter Schierack, Dirk Reinhold, Karsten Conrad, and Dirk Roggenbuck, “Next-Generation Autoantibody Testing by Combination of Screening and Confirmation—the CytoBead® Technology,” Clinical Reviews in Allergy & Immunology, 2016. View at Publisher · View at Google Scholar
  • M. Infantino, F. Meacci, V. Grossi, M. Manfredi, M. Benucci, M. Merone, and P. Soda, “The burden of the variability introduced by the HEp-2 assay kit and the CAD system in ANA indirect immunofluorescence test,” Immunologic Research, 2016. View at Publisher · View at Google Scholar
  • Santa Di Cataldo, Simone Tonti, Andrea Bottino, and Elisa Ficarra, “ANAlyte: a modular image analysis tool for ANA testing with Indirect Immunofluorescence.,” Computer Methods and Programs in Biomedicine, 2016. View at Publisher · View at Google Scholar
  • Thomas Bahmer, Micaela Romagnoli, Francesco Girelli, Martin Claussen, and Klaus F. Rabe, “The use of auto-antibody testing in the evaluation of interstitial lung disease (ILD) A practical approach for the pulmonologist,” Respiratory Medicine, 2016. View at Publisher · View at Google Scholar
  • Mohammed Alsuwaidi, Margit Dollinger, Martin Fleck, and Boris Ehrenstein, “The Reliability of a Novel Automated System for ANA Immunofluorescence Analysis in Daily Clinical Practice,” International Journal of Rheumatology, vol. 2016, pp. 1–5, 2016. View at Publisher · View at Google Scholar
  • Ralf J. Ludwig, Karen Vanhoorelbeke, Frank Leypoldt, Ziya Kaya, Katja Bieber, Sandra M. McLachlan, Lars Komorowski, Jie Luo, Otavio Cabral-Marques, Christoph M. Hammers, Jon M. Lindstrom, Peter Lamprecht, Andrea Fischer, Gabriela Riemekasten, Claudia Tersteeg, Peter Sondermann, Basil Rapoport, Klaus-Peter Wandinger, Christian Probst, Asmaa El Beidaq, Enno Schmidt, Alan Verkman, Rudolf A. Manz, and Falk Nimmerjahn, “Mechanisms of Autoantibody-Induced Pathology,” Frontiers in Immunology, vol. 8, 2017. View at Publisher · View at Google Scholar
  • Anne E. Tebo, “Recent approaches to optimize laboratory assessment of antinuclear antibodies,” Clinical and Vaccine Immunology, vol. 24, no. 12, 2017. View at Publisher · View at Google Scholar
  • Melanie Hahn, Stefan Gerlach, Joyce Giesen, Kai Fechner, Jan G.M.C. Damoiseaux, Wymke Hormann, Nicola Hochstrate, and Kai Affeldt, “Performance analysis of automated evaluation of Crithidia luciliae-based indirect immunofluorescence tests in a routine setting - Strengths and weaknesses,” Clinical Chemistry and Laboratory Medicine, vol. 56, no. 1, pp. 86–93, 2017. View at Publisher · View at Google Scholar
  • Jingbo Lu, Xinming Shi, Jie Zhang, Min Li, Bing Zheng, Enling Li, and Haoming Zhu, “Automated antinuclear immunofluorescence antibody analysis is a reliable approach in routine clinical laboratories,” Clinical Chemistry and Laboratory Medicine, vol. 55, no. 12, pp. 1922–1930, 2017. View at Publisher · View at Google Scholar
  • A. Rigon, M. Infantino, M. Merone, G. Iannello, A. Tincani, I. Cavazzana, N. Caraballese, A. Radice, M. Manfredi, P. Soda, and A. Afeltra, “The inter-observer reading variability in anti-nuclear antibodies indirect (ANA) immunofluorescence test: a multicenter evaluation and a review of the literature,” Autoimmunity Reviews, 2017. View at Publisher · View at Google Scholar
  • In Young Yoo, Jong Won Oh, Hoon-Suk Cha, Eun-Mi Koh, and Eun-Suk Kang, “Performance of an Automated Fluorescence Antinuclear Antibody Image Analyzer,” Annals of Laboratory Medicine, vol. 37, no. 3, pp. 240, 2017. View at Publisher · View at Google Scholar
  • Joyce J. B. C. van Beers, Melanie Hahn, Johanna Fraune, Kathleen Mallet, Christopher Krause, Wymke Hormann, Kai Fechner, and Jan G. M. C. Damoiseaux, “Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting,” Autoimmunity Highlights, vol. 9, no. 1, 2018. View at Publisher · View at Google Scholar
  • ZhiYan Li, RuiLin Han, ZhenLin Yan, LiJuan Li, and ZhenRu Feng, “Antinuclear antibodies detection: A comparative study between automated recognition and conventional visual interpretation,” Journal of Clinical Laboratory Analysis, pp. e22619, 2018. View at Publisher · View at Google Scholar
  • Vincent Ricchiuti, Joseph Adams, Donna J. Hardy, Alexander Katayev, and James K. Fleming, “Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing,” Frontiers in Immunology, vol. 9, 2018. View at Publisher · View at Google Scholar
  • Jinmi Kim, Woonhyoung Lee, Geun-Tae Kim, Hyon-Suk Kim, Soyoung Ock, In-Soo Kim, and Seri Jeong, “Diagnostic utility of automated indirect immunofluorescence compared to manual indirect immunofluorescence for anti-nuclear antibodies in patients with systemic rheumatic diseases: A systematic review and meta-analysis,” Seminars in Arthritis and Rheumatism, 2018. View at Publisher · View at Google Scholar
  • Teresa Carbone, Michele Gilio, Maria Carmela Padula, Giuseppina Tramontano, Salvatore D'Angelo, and Vito Pafundi, “A step towards standardization: A method for end-point titer determination by fluorescence index of an automated microscope. End-point titer determination by fluorescence index,” Journal of Immunological Methods, 2018. View at Publisher · View at Google Scholar