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Clinical and Developmental Immunology
Volume 2013, Article ID 926937, 7 pages
http://dx.doi.org/10.1155/2013/926937
Research Article

LPS-Induced Inflammation Potentiates the IL-1 -Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

1Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, 05-110 Jabłonna, Poland
2The Academy of Cosmetics and Health Care, 13 Podwale Street, 00-252 Warsaw, Poland

Received 9 May 2013; Revised 20 June 2013; Accepted 2 July 2013

Academic Editor: Carlos Barcia

Copyright © 2013 Andrzej Przemysław Herman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acting at the level of the brain, interleukin- (IL-)1β is considered to be one of the most potent downregulators of reproduction processes during immune/inflammatory challenge. IL-1β suppresses gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus resulting in the inhibition of the luteinizing hormone (LH) release from the anterior pituitary (AP). However, the presence of IL-1β receptors in the AP suggests the possible direct action of this cytokine on LH secretion. The study was designed to determine the effect of IL-1β on the LH secretion from the AP explants collected from saline and LPS-treated ewes in the follicular phase. It was found that IL-1β suppressed ( ) GnRH-stimulated LH release and LHβ gene expression in AP explants in both groups. However, IL-1β action was more potent in the explants collected from LPS-treated animals. Pituitaries from LPS-treated animals were characterized by increased ( ) IL-1 type I receptor and decreased ( ) GnRH receptor gene expression level compared to the saline-treated group. IL-1β also affected the GnRH-R gene expression in explants collected from LPS-treated animals. Our results show that direct action of IL-1β on the pituitary gonadotropes could be one of the reasons of the reproductive processes disorders accompanying an inflammatory state.