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Clinical and Developmental Immunology
Volume 2013, Article ID 952469, 6 pages
http://dx.doi.org/10.1155/2013/952469
Research Article

Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain

1Laboratory for Thrombosis Research, Interdisciplinary Research Facility Life Sciences Kulak, E. Sabbelaan 53, 8500 Kortrijk, Belgium
2Department of Neurosciences, Laboratory of Experimental Neurosurgery and Neuroanatomy, KU Leuven, Herestraat 49, ON1, Box 811, 3000 Leuven, Belgium
3Laboratory of Pediatric Immunology, Department of Microbiology and Immunology, KU Leuven, Herestraat 49, ON1, Box 811, 3000 Leuven, Belgium
4Bioceros B.V., Alexander Numan Building 2nd floor, Yalelaan 46, 3584 CM Utrecht, The Netherlands

Received 1 February 2013; Revised 2 April 2013; Accepted 3 April 2013

Academic Editor: Mohamad Mohty

Copyright © 2013 Wim Maes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The recruitment and activation of regulatory T cells (Tregs) in the micro-environment of malignant brain tumors has detrimental effects on antitumoral immune responses. Hence, local elimination of Tregs within the tumor micro-environment represents a highly valuable tool from both a fundamental and clinical perspective. In the syngeneic experimental GL261 murine glioma model, Tregs were prophylactically eliminated through treatment with PC61, an anti-CD25 mAb. This resulted in specific elimination of CD4+CD25hiFoxp3+ Treg within brain-infiltrating lymphocytes and complete protection against subsequent orthotopic GL261 tumor challenge. Interestingly, PC61-treated mice also showed a pronounced infiltration of CD11b+ myeloid cells in the brain. Phenotypically, these cells could not be considered as Gr-1+ myeloid-derived suppressor cells (MDSC) but were identified as F4/80+ macrophages and granulocytes.