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Journal of Immunology Research
Volume 2015 (2015), Article ID 106743, 10 pages
http://dx.doi.org/10.1155/2015/106743
Research Article

Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells

1Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland
2Department of Regenerative Medicine and Immune Regulation, Medical University of Białystok, Waszyngtona 13 Street, 15-269 Białystok, Poland
3Faculty of Mechanical Engineering, University of Technology and Sciences in Bydgoszcz, Kaliskiego 7 Street, 85-789 Bydgoszcz, Poland
4The Institute of Technology Fermentation and Microbiology, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Wólczańska 171/173 Street, 90-924 Łódź, Poland
5Department of Clinical Microbiology and Immunology, Children’s Memorial Hospital, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland

Received 13 July 2014; Revised 11 September 2014; Accepted 15 September 2014

Academic Editor: Miguel Gueimonde

Copyright © 2015 Małgorzata Wiese et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.