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Journal of Immunology Research
Volume 2015, Article ID 120348, 14 pages
http://dx.doi.org/10.1155/2015/120348
Research Article

fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

1Laboratory of Molecular Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Independencia 631, 5110566 Valdivia, Chile
2Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Independencia 631, 5110566 Valdivia, Chile

Received 2 June 2015; Revised 30 September 2015; Accepted 7 October 2015

Academic Editor: Carlos Rosales

Copyright © 2015 María A. Hidalgo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In order to assess the effect of the inhibitors (DPI, HMAP, Amiloride, UO126, LY294002, SB203580 and Akt) on neutrophil viability, we used the Cell-Titer-Glo Luminescent Cell Viability Assay. We demonstrated that none inhibitors affected cellular viability, suggesting that the effect observed on the IL-8 release induced by fMLP, is not related with cytotoxicity.

  1. Supplementary Material