Infusion of Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate-Conjugated MOG35–55-Coupled Spleen Cells Effectively Prevents and Reverses Experimental Autoimmune Encephalomyelitis in Mice
Infusion of MOG-coupled spleen cells reverses clinical EAE. (a) Naïve C57BL/6 mice were immunized as in Figure 1. Around one week before EAE onset, mice were randomly divided into four groups (UV-MOG-SPs, MOG-SPs, SPs, or PBS control, 5 mice/group) and given 1 × 107 different cells or the same volume of PBS for 5 times (days 5, 10, 15, 20, and 23). The scores of treatment groups were compared to the scores of PBS treated group at each time point. ; . (b) A cohort of mice was monitored after EAE induction. When the clinical score reached 2 or above, the EAE mice were randomly divided into four groups (5 mice/group): UV-MOG-SPs, MOG-SPs, SPs, or PBS. The mice in different groups received corresponding treatments twice a week for three weeks. The scores of treatment groups were compared with the scores of PBS treated group at each time point. ; . (c) Sections from the spinal cords of EAE mice in late reversal experiments on day 50 (4 mice/group) were stained with H&E for inflammatory infiltrates (upper panel), LFB for demyelination (middle panel), and NF staining for axonal loss (bottom panel). Original magnification ×20. The arrows indicate areas of lesion with infiltrated cells, demyelination, and axonal loss. One representative animal of each group was exhibited. Similar results were obtained from two additional independent experiments.
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