MOG-coupled spleen cells treatment induces similar MOG-specific CD4+ T cell proliferation in all groups. Spleen cells from each group in Figure 3
were labeled with CFSE; then CFSE-labeled spleen cells (5 × 105
/well) were stimulated with MOG35–55
g/mL) or unrelated antigen KLH (10 μ
g/mL) for 4 days. Thereafter, the cells were harvested and stained with anti-CD4-PerCp. CD4+ T cell proliferation (dilution of CFSE) was examined by flow cytometry by gating CD4+ T cells. (a) The flow cytometric data on proliferating CD4+ T cells/total CD4+ T cells (%) from one representative animal of each group were shown. (b) The data on proliferating CD4+ T cells with CFSE dilution in all CFSE-labeled CD4+ T cells from all animals (10 mice/group) were summarized. Statistical analysis results were depicted in the figures. Similar data were obtained from two additional independent experiments.