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Journal of Immunology Research
Volume 2015, Article ID 217287, 10 pages
http://dx.doi.org/10.1155/2015/217287
Research Article

Multifunctional Analysis of CD4+ T-Cell Response as Immune-Based Model for Tuberculosis Detection

1Department of Public Health and Infectious Diseases, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy
2Infectious Diseases Unit, Sapienza University, Corso della Repubblica 79, 04100 Latina, Italy

Received 17 September 2014; Revised 30 December 2014; Accepted 30 December 2014

Academic Editor: Vishwanath Venketaraman

Copyright © 2015 Miriam Lichtner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. World Health Organization, Global Tuberculosis Control: WHO Report 2013, WHO, Geneva, Switzerland, 2013, http://www.who.int/tb/publications/global_report/en/.
  2. C. E. Barry Jr., H. I. Boshoff, V. Dartois et al., “The spectrum of latent tuberculosis: rethinking the biology and intervention strategies,” Nature Reviews Microbiology, vol. 7, no. 12, pp. 845–855, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. K. Dheda, S. K. Schwander, B. Zhu, R. N. Van Zyl-Smit, and Y. Zhang, “The immunology of tuberculosis: from bench to bedside,” Respirology, vol. 15, no. 3, pp. 433–450, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Pai, C. M. Denkinger, S. V. Kik et al., “Gamma interferon release assays for detection of Mycobacterium tuberculosis infection,” Clinical Microbiology Reviews, vol. 27, no. 1, pp. 3–20, 2014. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Sester, G. Sotgiu, C. Lange et al., “Interferon-γ release assays for the diagnosis of active tuberculosis: a systematic review and meta-analysis,” European Respiratory Journal, vol. 37, no. 1, pp. 100–111, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. J. Z. Metcalfe, C. K. Everett, K. R. Steingart et al., “Interferon-γ release assays for active pulmonary tuberculosis diagnosis in adults in low-and middle-income countries: systematic review and meta-analysis,” Journal of Infectious Diseases, vol. 204, no. 4, supplement, pp. S1120–S1129, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. J. S. Sutherland, I. M. Adetifa, P. C. Hill, R. A. Adegbola, and M. O. C. Ota, “Pattern and diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease,” European Journal of Immunology, vol. 39, no. 3, pp. 723–729, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. V. Sargentini, S. Mariotti, S. Carrara et al., “Cytometric detection of antigen-specific IFN-γ/IL-2 secreting cells in the diagnosis of tuberculosis,” BMC Infectious Diseases, vol. 9, article 99, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. N. Caccamo, G. Guggino, S. A. Joosten et al., “Multifunctional CD4+ T cells correlate with active Mycobacterium tuberculosis infection,” European Journal of Immunology, vol. 40, no. 8, pp. 2211–2220, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Harari, V. Rozot, F. B. Enders et al., “Dominant TNF-α+Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease,” Nature Medicine, vol. 17, no. 3, pp. 372–377, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Streitz, S. Fuhrmann, D. Thomas et al., “The phenotypic distribution and functional profile of tuberculin-specific CD4 T-cells characterizes different stages of TB infection,” Cytometry Part B, Clinical Cytometry, vol. 82, no. 6, pp. 360–368, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. W. L. Leung, K. L. Law, V. S. S. Leung et al., “Comparison of intracellular cytokine flow cytometry and an enzyme immunoassay for evaluation of cellular immune response to active tuberculosis,” Clinical and Vaccine Immunology, vol. 16, no. 3, pp. 344–351, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. D. I. Won and J. R. Park, “Flow cytometric measurements of TB-specific T cells comparing with quantiFERON-TB gold,” Cytometry Part B—Clinical Cytometry, vol. 78, no. 2, pp. 71–80, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. J. Lee, S. Y. Lee, D. I. Won, S. I. Cha, J. Y. Park, and C. H. Kim, “Comparison of whole-blood interferon-γ assay and flow cytometry for the detection of tuberculosis infection,” Journal of Infection, vol. 66, no. 4, pp. 338–345, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. E. Petruccioli, L. Petrone, V. Vanini et al., “IFNγ/TNFα specific-cells and effector memory phenotype associate with active tuberculosis,” Journal of Infection, vol. 66, no. 6, pp. 475–486, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. E. K. Forbes, C. Sander, E. O. Ronan et al., “Multifunctional, high-level cytokine-producing Th1 cells in the lung, but not spleen, correlate with protection against Mycobacterium tuberculosis aerosol challenge in mice,” The Journal of Immunology, vol. 181, no. 7, pp. 4955–4964, 2008. View at Google Scholar · View at Scopus
  17. C. L. Day, D. A. Abrahams, L. Lerumo et al., “Functional capacity of Mycobacterium tuberculosis-specific T cell responses in humans is associated with mycobacterial load,” The Journal of Immunology, vol. 187, no. 5, pp. 2222–2232, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. B. M. Kagina, N. Mansoor, E. P. Kpamegan et al., “Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry,” Journal of Immunological Methods, vol. 22, no. 14, pp. 356–361, 2014. View at Publisher · View at Google Scholar
  19. S. Commandeur, K. E. Van Meijgaarden, C. Prins et al., “An unbiased genome-wide Mycobacterium tuberculosis gene expression approach to discover antigens targeted by human T cells expressed during pulmonary infection,” Journal of Immunology, vol. 190, no. 4, pp. 1659–1671, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. I. Sauzullo, R. Scrivo, F. Mengoni et al., “Multi-functional flow cytometry analysis of CD4+ T cells as an immune biomarker for latent tuberculosis status in patients treated with tumour necrosis factor (TNF) antagonists,” Clinical and Experimental Immunology, vol. 176, no. 3, pp. 410–417, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. R. Diel, D. Goletti, G. Ferrara et al., “Interferon-γ release assays for the diagnosis of latent Mycobacterium tuberculosis infection: a systematic review and meta-analysis,” European Respiratory Journal, vol. 37, no. 1, pp. 88–99, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. I. Sauzullo, F. Mengoni, R. Scrivo et al., “Evaluation of QuantiFERON-TB gold in-tube in human immunodeficiency virus infection and in patient candidates for anti-tumour necrosis factor-alpha treatment,” The International Journal of Tuberculosis and Lung Disease, vol. 14, no. 7, pp. 834–840, 2010. View at Google Scholar · View at Scopus
  23. M. Santin, L. Muñoz, and D. Rigau, “Interferon-gamma release assays for the diagnosis of tuberculosis and tuberculosis infection in HIV-infected adults: a systematic review and meta-analysis,” PLoS ONE, vol. 7, no. 3, Article ID e32482, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. R. Scrivo, I. Sauzullo, F. Mengoni et al., “Serial interferon-γ release assays for screening andmonitoring of tuberculosis infection during treatment with biologic agents,” Clinical Rheumatology, vol. 31, no. 11, pp. 1567–1575, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. R. Scrivo, I. Sauzullo, F. Mengoni et al., “Mycobacterial interferon-γ release variations during longterm treatment with tumor necrosis factor blockers: lack of correlation with clinical outcome,” The Journal of Rheumatology, vol. 40, no. 2, pp. 157–165, 2013. View at Publisher · View at Google Scholar · View at Scopus
  26. I. Sauzullo, F. Mengoni, R. Marocco et al., “Interferon-γ release assay for tuberculosis in patients with psoriasis treated with tumour necrosis factor antagonists: in vivo and in vitro analysis,” British Journal of Dermatology, vol. 169, no. 5, pp. 1133–1140, 2013. View at Publisher · View at Google Scholar · View at Scopus
  27. K. H. Kim, S. W. Lee, W. T. Chung et al., “Serial interferon-gamma release assays for the diagnosis of latent tuberculosis infection in patients treated with immunosuppressive agents,” The Korean Journal of Laboratory Medicine, vol. 31, no. 4, pp. 271–278, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. S. Kannanganat, C. Ibegbu, L. Chennareddi, H. L. Robinson, and R. R. Amara, “Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells,” Journal of Virology, vol. 81, no. 16, pp. 8468–8476, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. J. T. Mattila, C. R. Diedrich, P. Ling Lin, J. Phuah, and J. L. Flynn, “Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation,” Journal of Immunology, vol. 186, no. 6, pp. 3527–3537, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. K. A. Millington, J. A. Innes, S. Hackforth et al., “Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load,” The Journal of Immunology, vol. 178, no. 8, pp. 5217–5226, 2007. View at Publisher · View at Google Scholar · View at Scopus
  31. K. A. Millington, S. Gooding, T. S. C. Hinks, D. J. M. Reynolds, and A. Lalvani, “Mycobacterium tuberculosis-specific cellular immune profiles suggest bacillary persistence decades after spontaneous cure in untreated tuberculosis,” The Journal of Infectious Diseases, vol. 202, no. 11, pp. 1685–1689, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Streitz, S. Fuhrmann, F. Powell et al., “Tuberculin-specific T cells are reduced in active pulmonary tuberculosis compared to LTBI or status post BCG vaccination,” The Journal of Infectious Diseases, vol. 203, no. 3, pp. 378–382, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. C. H. Kim, K. J. Choi, S. S. Yoo et al., “Comparative analysis of whole-blood interferon-γ and flow cytometry assays for detecting post-treatment Immune responses in patients with active tuberculosis,” Cytometry B: Clinical Cytometry, vol. 86, no. 4, pp. 236–243, 2014. View at Publisher · View at Google Scholar
  34. G. Pantaleo and A. Harari, “Functional signatures in antiviral T-cell immunity for monitoring virus-associated diseases,” Nature Reviews Immunology, vol. 6, no. 5, pp. 417–423, 2006. View at Publisher · View at Google Scholar · View at Scopus
  35. M. R. Betts, M. C. Nason, S. M. West et al., “HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells,” Blood, vol. 107, no. 12, pp. 4781–4789, 2006. View at Publisher · View at Google Scholar · View at Scopus
  36. V. Rozot, S. Vigano, J. Mazza-Stalder et al., “Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease,” European Journal of Immunology, vol. 43, no. 6, pp. 1568–1577, 2013. View at Publisher · View at Google Scholar · View at Scopus
  37. V. Rozot, A. Patrizia, and S. Vigano, “Combined use of Mycobacterium tuberculosis-specific CD4 and CD8 T-cell responses is a powerful diagnostic tool of active tuberculosis,” Clinical of Infectious Diseases, vol. 60, no. 3, pp. 432–437, 2015. View at Publisher · View at Google Scholar
  38. D. A. Lewinsohn, A. S. Heinzel, J. M. Gardner, L. Zhu, M. R. Alderson, and D. M. Lewinsohn, “Mycobacterium tuberculosis-specific CD8+ T cells preferentially recognize heavily infected cells,” The American Journal of Respiratory and Critical Care Medicine, vol. 168, no. 11, pp. 1346–1352, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. C. Lancioni, M. Nyendak, S. Kiguli et al., “CD8+ T cells provide an immunologic signature of tuberculosis in young children,” The American Journal of Respiratory and Critical Care Medicine, vol. 185, no. 2, pp. 206–212, 2012. View at Publisher · View at Google Scholar · View at Scopus