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Journal of Immunology Research
Volume 2015, Article ID 323506, 9 pages
Research Article

Utilities for High-Throughput Analysis of B-Cell Clonal Lineages

Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK

Received 1 June 2015; Accepted 12 July 2015

Academic Editor: Guanglan Zhang

Copyright © 2015 William D. Lees and Adrian J. Shepherd. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


There are at present few tools available to assist with the determination and analysis of B-cell lineage trees from next-generation sequencing data. Here we present two utilities that support automated large-scale analysis and the creation of publication-quality results. The tools are available on the web and are also available for download so that they can be integrated into an automated pipeline. Critically, and in contrast to previously published tools, these utilities can be used with any suitable phylogenetic inference method and with any antibody germline library and hence are species-independent.