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Journal of Immunology Research
Volume 2015 (2015), Article ID 361604, 12 pages
Research Article

Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota

1Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, France
2Cátedra de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115 s/n, 1900 La Plata, Argentina
3Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), CCT La Plata-CONICET, UNLP, 47 y 115 s/n, 1900 La Plata, Argentina

Received 18 July 2014; Revised 22 September 2014; Accepted 23 September 2014

Academic Editor: Borja Sánchez

Copyright © 2015 P. Carasi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders.