In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer

Figure 3

Comparison of direct NK- or indirect NK- recognition of 4 HER2 positive cell lines. Cytotoxicity was evaluated from release after 4 hours of incubation; the data represent the means of three independent experiments. For ADCC, cell lines were incubated in the presence of 10 μg/mL trastuzumab. In the absence of antibody BT474, MDA-MB-453 and MCF7 were not sensitive to NK activity of NK-92 (see Figure 2 and data are not shown for MCF7).

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