Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells

Figure 3

No difference in cytokine secretion between mock- and MelanA-RNA-electroporated DC. DCs were electroporated either without RNA (Mock) or with a panel of 16 RNAs encoding different tumor antigens, either mutated or not, and either linked to the lysosomal targeting signal DC-LAMP or not (see Table 1). Twenty-four hours after electroporation, supernatants of the cells were taken and the cytokine secretion by the cells was determined in a cytometric bead array. Cytokine secretion of mock-electroporated DCs was defined as 100% and concentrations after electroporation of the mRNAs were put in relation to that. Shown are averages of at least 3 independent experiments. Error bars indicate the standard deviation (SD). According to a 1-way ANOVA with multiple comparison test, no statistically significant differences were present within the cytokine secretion data ().