Early Differentiation of Human CD11c⁺NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

Figure 4

Preferential production of functional CD11c⁺CD16⁺ NK-like cells promoted by hematopoietic-stromal cell communication responding to DLE. MS-5 stromal cells were exposed or not to DLE Transferon 24 hours before coculturing with DLE Transferon-pretreated CD34⁺ hematopoietic stem and progenitor cells (HSPC), as described in Figure 3(a). All conditions were placed in lymphoid lineage cocultures for 30 days followed by flow cytometry analyses, where the indicated gates were used to discriminate CD56⁺CD11c⁺ and CD56⁺CD16⁺CD11c⁺ NK cell frequencies (a). Yields per input progenitor were tabulated to record the significant variations by stromal or hematopoietic cells exposure to DLE Transferon (b). .

(a)

(b)