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Journal of Immunology Research
Volume 2016, Article ID 5457010, 11 pages
Research Article

In Vitro Effects of Some Botanicals with Anti-Inflammatory and Antitoxic Activity

1Division of Research and Development, SANYpet SpA, 35023 Bagnoli di Sopra, Italy
2School of Specialization in Clinical Biochemistry, “G. d’Annunzio” University, 66100 Chieti, Italy
3Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy
4Ph.D. School of Science, University of Basilicata, 85100 Potenza, Italy
5Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80100 Naples, Italy
6Department of Science, University of Basilicata, 85100 Potenza, Italy

Received 31 May 2016; Revised 19 July 2016; Accepted 21 July 2016

Academic Editor: Giuseppe A. Sautto

Copyright © 2016 Gianandrea Guidetti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Several extrinsic factors, like drugs and chemicals, can foster autoimmunity. Tetracyclines, in particular oxytetracycline (OTC), appear to correlate with the emergence of immune-mediated diseases. Accumulation of OTC, the elective drug for gastrointestinal and respiratory infectious disease treatment in broiler chickens, was reported in chicken edible tissues and could represent a potential risk for pets and humans that could assume this antibiotic as residue in meat or in meat-derived byproducts. We investigated the in vitro anti-inflammatory properties of a pool of thirteen botanicals as a part of a nutraceutical diet, with proven immunomodulatory activity. In addition, we evaluated the effect of such botanicals in contrasting the in vitro proinflammatory toxicity of OTC. Our results showed a significant reduction in interferon- (INF-) γ production by human and canine lymphocytes in presence of botanicals (). Increased INF-γ production, dependent on 24-hour OTC-incubation of T lymphocytes, was significantly reduced by the coincubation with Haematococcus pluvialis, with Glycine max, and with the mix of all botanicals (). In conclusion, the use of these botanicals was shown to be able to contrast OTC-toxicity and could represent a new approach for the development of functional foods useful to enhance the standard pharmacological treatment in infections as well as in preventing or reducing the emergence of inflammatory diseases.