Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles

Figure 3

Effect of VP6 nanotubes on RAW 264.7 cell surface markers expression. The expression of CD40, CD80, CD86, and MHC class II (I-A/I-E) was analyzed by flow cytometry after 24 h and 48 h incubation in the presence of 50 μg/mL VP6, 1 : 10 diluted RV Wa, 1 μg/mL LPS, or culture media (CM) only. (a) Expression of the molecules on the cells in CM only and upregulation induced by VP6 and RV Wa are shown by overlaid histograms. The results are representative of three independent analyses done. (b) Table of mean fluorescence intensity (MFI) values obtained from the histograms in Figure 3(a) of each cell surface marker after treatment with VP6, RV Wa, LPS, or untreated cells.

(a)

(b)