Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils

Figure 3

Effect of STK and Syk inhibition on inflammatory cytokine gene expression in human neutrophils. Cells were pretreated with STK or Syk inhibitors, prior to a 30 min stimulation with LPS, TNFα, fMLP, or GM-CSF, as described for Figure 2. Total RNA was isolated, reverse-transcribed, and analyzed for cytokine gene expression by real-time qPCR. Values were normalized over RPL32 and are represented as fold increase relative to unstimulated cells. Mean ± SEM from at least 3 independent experiments, each performed in duplicate. §, versus unstimulated cells (“ctrl”).