Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils
Effect of STK and Syk inhibition on inflammatory cytokine gene expression in human neutrophils. Cells were pretreated with STK or Syk inhibitors, prior to a 30 min stimulation with LPS, TNFα, fMLP, or GM-CSF, as described for Figure 2. Total RNA was isolated, reverse-transcribed, and analyzed for cytokine gene expression by real-time qPCR. Values were normalized over RPL32 and are represented as fold increase relative to unstimulated cells. Mean ± SEM from at least 3 independent experiments, each performed in duplicate. §, versus unstimulated cells (“ctrl”).