Table of Contents Author Guidelines Submit a Manuscript
Journal of Immunology Research
Volume 2017 (2017), Article ID 5871043, 10 pages
https://doi.org/10.1155/2017/5871043
Research Article

Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis

1Basic Pathology Department, Federal University of Paraná, Curitiba, PR, Brazil
2Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil

Correspondence should be addressed to Vanete Thomaz Soccol; moc.liamg@loccosetenav

Received 26 September 2016; Revised 20 November 2016; Accepted 8 December 2016; Published 7 March 2017

Academic Editor: Kristen M. Kahle

Copyright © 2017 Juliana Seger Link et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Desjeux, “Leishmaniasis: current situation and new perspectives,” Comparative Immunology, Microbiology and Infectious Diseases, vol. 27, no. 5, pp. 305–318, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. J. Alvar, I. D. Vélez, C. Bern et al., “Leishmaniasis worldwide and global estimates of its incidence,” PLoS ONE, vol. 7, no. 5, Article ID e35671, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Szargiki, E. A. de Castro, E. Luz, W. Kowalthuk, Â. M. Machado, and V. Thomaz-Soccol, “Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil,” Brazilian Journal of Infectious Diseases, vol. 13, no. 1, pp. 47–52, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. M. de Paiva-Cavalcanti, R. C. S. de Morais, R. Pessoa-e-Silva et al., “Leishmaniases diagnosis: an update on the use of immunological and molecular tools,” Cell and Bioscience, vol. 5, no. 1, article 31, 2015. View at Publisher · View at Google Scholar · View at Scopus
  5. W. R. Faber, L. Oskam, T. Van Gool et al., “Value of diagnostic techniques for cutaneous leishmaniasis,” Journal of the American Academy of Dermatology, vol. 49, no. 1, pp. 70–74, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. E. Nuzum, F. White, C. Thakur et al., “Diagnosis of symptomatic visceral leishmaniasis by use of the polymerase chain reaction on patient blood,” Journal of Infectious Diseases, vol. 171, no. 3, pp. 751–754, 1995. View at Publisher · View at Google Scholar · View at Scopus
  7. Y. Le Fichoux, J.-F. Quaranta, J.-P. Aufeuvre et al., “Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France,” Journal of Clinical Microbiology, vol. 37, no. 6, pp. 1953–1957, 1999. View at Google Scholar · View at Scopus
  8. L. Lachaud, J. Dereure, E. Chabbert et al., “Optimized PCR using patient blood samples for diagnosis and follow-up of visceral leishmaniasis, with special reference to AIDS patients,” Journal of Clinical Microbiology, vol. 38, no. 1, pp. 236–240, 2000. View at Google Scholar · View at Scopus
  9. J. Montenegro, “Cutaneous reaction in leishmaniasis,” Archives of Dermatology and Syphilology, vol. 13, no. 2, pp. 187–194, 1926. View at Publisher · View at Google Scholar · View at Scopus
  10. K. A. G. Yoneyama, L. D. de Peder, M. V. C. Lonardoni, and T. G. V. Silveira, “Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil,” Brazilian Journal of Infectious Diseases, vol. 11, no. 3, pp. 360–364, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. D. Menezes-Souza, T. A. de Oliveira Mendes, A. C. de Araújo Leão, M. de Souza Gomes, R. T. Fujiwara, and D. C. Bartholomeu, “Linear B-cell epitope mapping of MAPK3 and MAPK4 from Leishmania braziliensis: implications for the serodiagnosis of human and canine leishmaniasis,” Applied Microbiology and Biotechnology, vol. 99, no. 3, pp. 1323–1336, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. M. A. Chávez-Fumagalli, V. T. Martins, M. C. S. Testasicca et al., “Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach,” Clinical and Vaccine Immunology: CVI, vol. 20, no. 6, pp. 835–841, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Seyed, F. Zahedifard, S. Safaiyan et al., “In silico analysis of six known Leishmania major antigens and in vitro evaluation of specific epitopes eliciting HLA-A2 restricted CD8 T cell response,” PLoS Neglected Tropical Diseases, vol. 5, no. 9, Article ID e1295, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. A. Duarte, A. T. L. Queiroz, R. Tosta et al., “Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: in silico search and in vivo validation,” PLoS ONE, vol. 10, no. 4, Article ID e0124786, 2015. View at Publisher · View at Google Scholar · View at Scopus
  15. G. G. S. Oliveira, F. B. Magalhães, M. C. A. Teixeira et al., “Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis,” American Journal of Tropical Medicine and Hygiene, vol. 85, no. 6, pp. 1025–1034, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. S. Kumar, D. Kumar, J. Chakravarty, M. Rai, and S. Sundar, “Identification and characterization of a novel Leishmania donovani antigen for serodiagnosis of visceral leishmaniasis,” American Journal of Tropical Medicine and Hygiene, vol. 86, no. 4, pp. 601–605, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. S. F. Parmley and G. P. Smith, “Antibody-selectable filamentous fd phage vectors: affinity purification of target genes,” Gene, vol. 73, no. 2, pp. 305–318, 1988. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Capelli-Peixoto, C. Chávez-Olórtegui, D. Chaves-Moreira et al., “Evaluation of the protective potential of a Taenia solium cysticercus mimotope on murine cysticercosis,” Vaccine, vol. 29, no. 51, pp. 9473–9479, 2011. View at Publisher · View at Google Scholar · View at Scopus
  19. S. M. Alban, J. F. de Moura, J. C. Minozzo, M. T. Mira, and V. T. Soccol, “Identification of mimotopes of Mycobacterium leprae as potential diagnostic reagents,” BMC Infectious Diseases, vol. 13, no. 42, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. S. M. Alban, J. F. De Moura, V. Thomaz-Soccol et al., “Phage display and synthetic peptides as promising biotechnological tools for the serological diagnosis of leprosy,” PLoS ONE, vol. 9, no. 8, Article ID e106222, 7 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. R. L. Fogaça, J. Capelli-Peixoto, I. B. Yamanaka et al., “Phage-displayed peptides as capture antigens in an innovative assay for Taenia saginata-infected cattle,” Applied Microbiology and Biotechnology, vol. 98, no. 21, pp. 8887–8894, 2014. View at Publisher · View at Google Scholar · View at Scopus
  22. C. M. Toledo-Machado, L. L. Bueno, D. Menezes-Souza et al., “Use of Phage Display technology in development of canine visceral leishmaniasis vaccine using synthetic peptide trapped in sphingomyelin/cholesterol liposomes,” Parasites and Vectors, vol. 8, no. 1, article no. 133, 2015. View at Publisher · View at Google Scholar · View at Scopus
  23. B. Autran, G. Carcelain, B. Combadiere, and P. Debre, “Therapeutic vaccines for chronic infections,” Science, vol. 305, no. 5681, pp. 205–208, 2004. View at Publisher · View at Google Scholar · View at Scopus
  24. I. D. Vélez, K. Gilchrist, S. Martínez et al., “Safety and immunogenicity of a defined vaccine for the prevention of cutaneous leishmaniasis,” Vaccine, vol. 28, no. 2, pp. 329–337, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  26. R. C. Ribas-Silva, T. R. Navasconi, L. de Souza Braga et al., “Serological and molecular investigation of cutaneous leishmaniasis in healthy individuals from an American Cutaneous Leishmaniasis-endemic region,” American Journal of Infectious Diseases, vol. 11, no. 1, pp. 20–25, 2015. View at Publisher · View at Google Scholar · View at Scopus
  27. E. Harlow and D. Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998.
  28. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar · View at Scopus
  29. L. L. C. Bonnycastle, J. S. Mehroke, M. Rashed, X. Gong, and J. K. Scott, “Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage,” Journal of Molecular Biology, vol. 258, no. 5, pp. 747–762, 1996. View at Publisher · View at Google Scholar · View at Scopus
  30. C. E. Metz, “Basic principles of ROC analysis,” Seminars in Nuclear Medicine, vol. 8, no. 4, pp. 283–298, 1978. View at Publisher · View at Google Scholar · View at Scopus
  31. M. H. Zweig and G. Campbell, “Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine,” Clinical Chemistry, vol. 39, no. 4, pp. 561–577, 1993. View at Google Scholar · View at Scopus
  32. V. Thomaz-Soccol, S. M. Alban, and J. F. Moura, “Uso de peptídeos miméticos de Mycobacterium leprae para diagnóstico e vacinas,” Brazilian Patent PI015110000997, 2011.
  33. V. Thomaz-Soccol, S. M. Alban, and J. Seger, “Peptídeos miméticos de Leishmania sp. Processo para sua obtenção e aplicações,” Brazilian Patent 10200150177240, 2015.
  34. J. de Moura, L. M. Alvarenga, and V. Thomaz-Soccol, “Biotechnological role of phage-displayed peptides for the diagnosis of neglected tropical diseases,” Human and Animal Health Applications, vol. 9, pp. 161–180, 2017. View at Google Scholar
  35. R. Etges, J. Bouvier, and C. Bordier, “The major surface protein of Leishmania promastigotes is a protease,” Journal of Biological Chemistry, vol. 261, no. 20, pp. 9098–9101, 1986. View at Google Scholar · View at Scopus
  36. P. B. Joshi, B. L. Kelly, S. Kamhawi, D. L. Sacks, and W. R. McMaster, “Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor,” Molecular and Biochemical Parasitology, vol. 120, no. 1, pp. 33–40, 2002. View at Publisher · View at Google Scholar · View at Scopus
  37. G. Gupta, S. Oghumu, and A. R. Satoskar, “Mechanisms of immune evasion in leishmaniasis,” Advances in Applied Microbiology, vol. 82, pp. 155–184, 2013. View at Publisher · View at Google Scholar · View at Scopus
  38. A. S. Hey, T. G. Theander, L. Hviid, S. M. Hazrati, M. Kemp, and A. Kharazmi, “The major surface glycoprotein (gp63) from Leishmania major and Leishmania donovani cleaves CD4 molecules on human T cells,” Journal of Immunology, vol. 152, no. 9, pp. 4542–4548, 1994. View at Google Scholar · View at Scopus
  39. M. R. Garcia, S. Graham, R. A. Harris, S. M. Beverley, and P. M. Kaye, “Epitope cleavage by Leishmania endopeptidase(s) limits the efficiency of the exogenous pathway of major histocompatibility complex class I-associated antigen presentation,” European Journal of Immunology, vol. 27, no. 4, pp. 1005–1013, 1997. View at Publisher · View at Google Scholar · View at Scopus
  40. N. D. Connell, E. Medina-Acosta, W. R. Mcmaster, B. R. Bloom, and D. G. Russell, “Effective immunization against cutaneous leishmaniasis with recombinant bacille Calmette-Guérin expressing the Leishmania surface proteinase gp63,” Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 24, pp. 11473–11477, 1993. View at Publisher · View at Google Scholar · View at Scopus
  41. G. R. Habibi, A. Khamesipour, W. R. McMaster, and F. Mahboudi, “Cytokine gene expression in healing and non-healing cases of cutaneous leishmaniasis in response to in vitro stimulation with recombinant gp63 using semi-quantitative RT-PCR,” Scandinavian Journal of Immunology, vol. 54, no. 4, pp. 414–420, 2001. View at Publisher · View at Google Scholar · View at Scopus
  42. M. R. Jaafari, A. Ghafarian, A. Farrokh-Gisour et al., “Immune response and protection assay of recombinant major surface glycoprotein of Leishmania (rgp63) reconstituted with liposomes in BALB/c mice,” Vaccine, vol. 24, no. 29-30, pp. 5708–5717, 2006. View at Publisher · View at Google Scholar · View at Scopus
  43. S. Bhowmick, R. Ravindran, and N. Ali, “Gp63 in stable cationic liposomes confers sustained vaccine immunity to susceptible BALB/c mice infected with Leishmania donovani,” Infection and Immunity, vol. 76, no. 3, pp. 1003–1015, 2008. View at Publisher · View at Google Scholar · View at Scopus
  44. R. Reithinger, J.-C. Dujardin, H. Louzir, C. Pirmez, B. Alexander, and S. Brooker, “Cutaneous leishmaniasis,” Lancet Infectious Diseases, vol. 7, no. 9, pp. 581–596, 2007. View at Publisher · View at Google Scholar · View at Scopus
  45. F. L. Schuster and J. J. Sullivan, “Cultivation of clinically significant hemoflagellates,” Clinical Microbiology Reviews, vol. 15, no. 3, pp. 374–389, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. A. Somanna, V. Mundodi, and L. Gedamu, “In vitro cultivation and characterization of Leishmania chagasi amastigote-like forms,” Acta Tropica, vol. 83, no. 1, pp. 37–42, 2002. View at Publisher · View at Google Scholar · View at Scopus
  47. A. R. Faria, M. M. Costa, M. S. Giusta et al., “High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis,” PLoS Neglected Tropical Diseases, vol. 5, no. 9, Article ID e1310, 2011. View at Publisher · View at Google Scholar · View at Scopus
  48. R. Porrozzi, M. V. Santos Da Costa, A. Teva et al., “Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs,” Clinical and Vaccine Immunology, vol. 14, no. 5, pp. 544–548, 2007. View at Publisher · View at Google Scholar · View at Scopus
  49. S. Passos, L. P. Carvalho, G. Orge et al., “Recombinant Leishmania antigens for serodiagnosis of visceral leishmaniasis,” Clinical and Diagnostic Laboratory Immunology, vol. 12, no. 10, pp. 1164–1167, 2005. View at Publisher · View at Google Scholar · View at Scopus
  50. M. M. Costa, M. Penido, M. S. Santos et al., “Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay,” PLoS Neglected Tropical Diseases, vol. 6, no. 5, Article ID e1622, 9 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  51. R. Badaró, D. Benson, M. C. Eulálio et al., “rK39: A cloned antigen of Leishmania chagasi that predicts active visceral leishmaniasis,” The Journal of Infectious Diseases, vol. 173, no. 3, pp. 758–761, 1996. View at Publisher · View at Google Scholar · View at Scopus