Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway

Table 1

Primer sequences used in qRT-PCR.

Genes	Accession numbers	Primer sequence (5→3)	Product size (bp)
IL-1β	NM_008361.3	Forward primer GCCACCTTTTGACAGTGATGAG	165
		Reverse primer AGTGATACTGCCTGCCTGAAG
IL-6	NM_031168.1	Forward primer CAACGATGATGCACTTGCAGA	201
		Reverse primer TCTCTCTGAAGGACTCTGGCT
TNF-α	NM_001278601.1	Forward primer ACCTGGCCTCTCTACCTTGT	161
		Reverse primer CCCGTAGGGCGATTACAGTC
iNOS	NM_010927.4	Forward primer AGGGACTGAGCTGTTAGAGACA	156
		Reverse primer AAGAGAAACTTCCAGGGGCAAG
GAPDH	NM_001289726.1	Forward primer GGTGAAGGTCGGTGTGAACG	232
		Reverse primer CCCGTAGGGCGATTACAGTC