Myeloperoxidase and Eosinophil Peroxidase Inhibit Endotoxin Activity and Increase Mouse Survival in a Lipopolysaccharide Lethal Dose 90% Model

Figure 1

(a) Regression plot of LPS standards expressed as time (sec) to maximum slope against mass of LPS (independent variable). For the range: 0.0012-5 ng lipopolysaccharide (LPS)/well the derived equation was with a coefficient of determination (²) of 0.982; for the range: 0.0012-0.625 ng LPS/well the equation was with an ² = 0.991. (b) Plots the percent (%) inhibition of the Limulus amebocyte lysate (LAL) activity for 5.0 ng LPS/well (the dependent variable, ) against varying concentrations (mg/well) of myeloperoxidase (the independent variable,). Testing was run in quadruplicate with all 60 data points depicted. The regression equation shows inhibition proportional to the natural log of the MPO mass with the ² of 0.9348. Additional statistical information is described in Table 1.

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