Figure 3: (a) Regression plot of lipid A standards (µg/well) against time (sec) to maximum slope with derived equations and 2 values. The top and bottom curves were obtained from the same set of standards 1.5- and 3.5-hour postpreparation of the lipid A standards as indicated. (b) Percent inhibition of Limulus amebocyte lysate (LAL) activity for 2.16 μg lipid A/well plotted against varying concentrations of MPO (filled circles) or EPO (open circles). The regression equations with 2 values for MPO and EPO inhibition of lipid A are shown. Lipid A standards were simultaneously run for each MPO or EPO inhibition experiment. Additional statistical information is described in Table 1.