Myeloperoxidase and Eosinophil Peroxidase Inhibit Endotoxin Activity and Increase Mouse Survival in a Lipopolysaccharide Lethal Dose 90% Model

Figure 4

Percent inhibition of lipopolysaccharide (LPS) endotoxin activity for a formulation composed of 4 μg myeloperoxidase (MPO) and 0.8 μg glucose oxidase (GO) with 23 μg Cl^-/well measured against varying concentrations of LPS using the Limulus amebocyte lysate (LAL) assay. The formulation was tested without D-glucose, i.e., no haloperoxidase activity (indicated by white boxes), and with a nonlimiting concentration (0.2 mg/well) of D-glucose, i.e., haloperoxidase activity (indicated by the black dots). The data points at 0.12 and 0.24 LPS show overlap and appear as black boxes. The regression equations with ² values show that the percentage inhibition of LPS endotoxin activity for a constant concentration of MPO plus GO is proportional to the negative log of the LPS concentration with or without D-glucose. Additional statistical information is described in Table 1.