Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope

Figure 6

Serum reactivity of mice immunized with the Mim_F8-1 phagotope. (a) Titers of serum IgG directed at phage proteins were determined by ELISA against immobilized Mim-F8 phagotope. (b) IgG binding reactivity of the sera (1 : 50 dilution) against immobilized BSA-W614A-3S was assayed by ELISA at day 53. BSA-3S-scr functioned as negative control. Binding activity is expressed as mean OD at 450 nm of duplicate wells and bars indicate SD. (c) Neutralizing activity of the sera (1 : 2 dilution) at day 53 was performed as mentioned in Figure 1 using the JR-CSF strain. Sera at day 0 were used as negative control. Experiments were run in duplicate and bars indicate SD.

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