MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of β-Catenin

Figure 4

Decreased MPC1 activates the WNT/β-catenin pathway by promoting nuclear translocation of β-catenin. (a) GSEA analysis of MPC1 expression in CRC using the TCGA dataset. NES: Normalized Enrichment Score. (b) Luciferase reporter gene assay of CRC cells treated with MPC1 overexpression or not. (c) The expression of total β-catenin and nuclear β-catenin was detected in control and MPC1-overexpression CRC cells, respectively. GAPDH and lamin A/C were used as the loading control of total and nuclear protein, respectively. (d) The gray value analysis of nuclear β-catenin in MPC1-overexpression cells and control cells. (e, f) MPC1 overexpression could inhibit the nuclear translocation of β-catenin in CRC cells. Scale bar: 100 μm. (g) IHC staining of β-catenin in mouse liver metastatic lesions inoculated with MC38 cells treatment with sh-MPC1 or control vector. Scale bar: 200 μm. (h, i) Relative mRNA expression level of β-catenin target genes in CRC cells with MPC1 overexpression or control vector. (j) Relative mRNA expression level of β-catenin target genes in MC38 cells with sh-MPC1 or control vector. (k–n) Relative protein expression level of β-catenin target genes in mouse liver tissue detected by IHC. Scale bar: 50 μm. Student’s -test, , .

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