Research Article

Improved Performance of ELISA and Immunochromatographic Tests Using a New Chimeric A2-Based Protein for Human Visceral Leishmaniasis Diagnosis

Figure 5

Titration curves for the determination of protein concentration and sera dilutions for DTL-4-ELISA. Pools of ten VL-positive and ten negative sera were assayed. (a) Titration of DTL-4 concentration by adding successive decreasing amounts of protein to wells on ELISA plates. (b) Titration of anti-DTL-4, anti-A2, and anti-K39 antibodies in pools of sera, using 10 ng of each protein per well.
(a)
(b)