Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 2

Comparative analysis by alignment of the amino acid sequences of several enolases: Haemophilus influenzae NTHiENO (ASO96723.1), Streptococcus pneumoniae (CTN02995.1), Trypanosoma cruzi (AGR66223.1), Entamoeba dispar (EDR27966.1), Candida albicans (AAA71939.1), Mus musculus (CAA40913.1), Homo sapiens β-enolase (NP_001967.3), Homo sapiens α-enolase (NP_001419.1), Klebsiella pneumoniae (SAW34188.1), Vibrio parahaemolyticus (KZW93652.1), Staphylococcus aureus (BBA23431.1), Streptococcus pyogenes (AKI77548.1), Streptococcus sobrinus (CAD60544.1); parentheses show GenBank accession numbers. The conserved amino acids in all sequences are labeled with asterisks; the conservative and semiconservative substitutions are labeled with two and one points, respectively. The dashed lines shown gaps introduced between the sequences. The residues involved in Mg²⁺ binding are shown in bold and with arrowhead, those forming the enolase signature are underlined, and the motifs for substrate binding are indicated with letters in bold. The putative plasminogen-binding site is boxed. The transmembranal domain is indicated in shadowed gray. The enolase “fingerprint” motif is boxed and shown with star. RNA-binding motifs are indicated with dashed bold lines over the motive. The percentage of sequence identity between NTHiENO and the enolases from other organisms is indicated at the end of figure.