Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 3

Evolutionary relationships of NTHiENO. The evolutionary history was inferred using the neighbor-joining method [56]. The bootstrap consensus tree inferred from 1000 replicates [57] is taken to represent the evolutionary history of the taxa analyzed [57]. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [57]. The evolutionary distances were computed using the Poisson correction method [58] and are in the units of the number of amino acid substitutions per site. This analysis involved 13 amino acid sequences. All ambiguous positions were removed for each sequence pair (pairwise deletion option). There were a total of 454 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [17].